Detailed information of TA system TA191879

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 147993..148667
• Replicon: plasmid unnamed3
• Accession: NZ_CP069846
• Organism: Klebsiella pneumoniae strain FDAARGOS_1305

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5Q9LMS5
• Locus tag: I6K35_RS01470
• Protein ID: WP_032720638.1
• Coordinates: 147993..148316 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6K35_RS01475
• Protein ID: WP_032720637.1
• Coordinates: 148365..148667 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K35_RS01450	143079..144425	-	1347	WP_077255522.1	ISNCY family transposase	-
I6K35_RS01455	144544..146076	+	1533	WP_085803464.1	IS3 family transposase	-
I6K35_RS01460	146334..146936	+	603	Protein_144	transposase	-
I6K35_RS01465	147263..147815	+	553	Protein_145	DUF4113 domain-containing protein	-
I6K35_RS01470	147993..148316	+	324	WP_032720638.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6K35_RS01475	148365..148667	+	303	WP_032720637.1	helix-turn-helix domain-containing protein	Antitoxin
I6K35_RS01480	148710..148877	-	168	WP_153591440.1	hypothetical protein	-
I6K35_RS01485	149065..149244	-	180	WP_032720701.1	hypothetical protein	-
I6K35_RS01490	149268..149651	-	384	WP_032720636.1	hypothetical protein	-
I6K35_RS01495	149756..150328	-	573	WP_032720635.1	GNAT family N-acetyltransferase	-
I6K35_RS01500	150330..151118	-	789	WP_032720700.1	TSUP family transporter	-
I6K35_RS01505	151154..152074	-	921	WP_050548604.1	TauD/TfdA family dioxygenase	-
I6K35_RS01510	152377..152871	-	495	WP_044266755.1	hypothetical protein	-
I6K35_RS01515	152902..153474	-	573	WP_044266753.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..241224	241224

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (45-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-83)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12372.29 Da
• Isoelectric Point: 9.6240

>T191879 WP_032720638.1 NZ_CP069846:147993-148316 [Klebsiella pneumoniae]
MNYLEFVETSAFSGLRKELMDDDEFRELQTYLLEAHDRGDTISHTGGCRKIRWGRPGMGKRGGVRVIYYVRLASGRLYLL
LIYPKNAKDELSEKEKAVMKALTQQLK

Nucleotide

• Length: 324 bp

>T191879 NZ_CP091977:3978526-3978810 [Aromatoleum aromaticum]
ATGGGGATCGTCTGGACGGAAGAGGCGATCGGCGACCTCGAAGAGATCCTCGCGTATTACTACGCCGAAGCGGGTCCGGC
AACGGCGCAGGCGGTCGAGGAACGCATCGTCGCCGAAATCATGGCGCTCACGACATTCCCCGAGAGGATCCGCACGAGCG
ACCGCGTCCCGGGCGCCCGGGAACTTGTGGTGCGACGCCTCCCATACGTGGTCTTCGTGAAAGGGGTCCCCGACGGCGTC
GTGGTGCTCAACGTGGTACACACGGCGAGGAAGTTTCCAGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11211.12 Da
• Isoelectric Point: 7.3611

>AT191879 WP_032720637.1 NZ_CP069846:148365-148667 [Klebsiella pneumoniae]
MEKKLFDALISSMEEMVAIENGQIQPAAEQVHRHTLPDVKAIRKHAGMKQAEFAEVVGASVDLVRSWEQQRRVPSGIALK
MLCLIEREPALINTLRGIHV

Nucleotide

• Length: 303 bp

>AT191879 NZ_CP091977:3978893-3979144 [Aromatoleum aromaticum]
ATGCATATATTGACGCATAATGACATCTTTACTGATGATGGTGTCCGCATGAGAACGACTGTCACGATCGATGATGATCT
GTACGAAAAGGCCCTGGAAGTGGCCGATCCGGCTATGGACAGGGCCGATCTCTTCCGCGAGGCGATGAAAACTTTCGTTC
GGGTACAAGCCGCCAAGCGCCTTGCTGCGTTGGGGGGCACCATGCCGGAGATGCAGGATGTGCCGCGACGGCGCGGCGAG
CCCTCGCAATGA

Structures

Toxin

• AlphaFold DB: A0A5Q9LMS5