Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1780080..1780698 Replicon chromosome
Accession NZ_CP069811
Organism Cupriavidus oxalaticus strain Ox1

Toxin (Protein)


Gene name graT Uniprot ID A0A375GMD0
Locus tag JTE92_RS07810 Protein ID WP_063240603.1
Coordinates 1780080..1780358 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A375GIG3
Locus tag JTE92_RS07815 Protein ID WP_063240604.1
Coordinates 1780369..1780698 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JTE92_RS07810 1780080..1780358 + 279 WP_063240603.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JTE92_RS07815 1780369..1780698 + 330 WP_063240604.1 HigA family addiction module antidote protein Antitoxin
JTE92_RS07820 1780788..1781384 - 597 WP_063240605.1 GTP cyclohydrolase II -
JTE92_RS07825 1781565..1782035 - 471 WP_063240606.1 Lrp/AsnC family transcriptional regulator -
JTE92_RS07830 1782188..1782550 + 363 WP_063240607.1 hypothetical protein -
JTE92_RS07835 1782560..1783477 - 918 WP_063240608.1 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(28-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-94)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10566.15 Da        Isoelectric Point: 8.5135

>T191831 WP_063240603.1 NZ_CP069811:1780080-1780358 [Cupriavidus oxalaticus]
MIQSFRCFDTAALFDGRYVARFASIEFIAQRKLEMLDDAATLGALRFPPGNRLEKLGGDRKGRYSIRINGQWRICFIWTH
DGPAAVEIIDYH

Download         Length: 279 bp

>T191831 NZ_CP091927:52267-52554 [Escherichia coli]
ATGACTTATACAGTAAAATTCCGGGATGATGCACTGAAAGAATGGCTCAGACTGGACAAGACCATTCAGCAGCAGTTCGC
AAAAAAACTGAAAAAATGCAGTGAAAATCCTCATATAGCTTCTGCAAAACTACGTGGATTAAAGGACTGCTATAAAATCA
AACTTCGCGCATCAGGTTTTCGTCTGGTTTATCAGATTATTGATGATATGTTAATTATTGCGGTCGTCGCAGTAGGTAAA
CGTGAACGCAGCAACGTTTATCATCTCGCCAGTGAACGGATGAAATAA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 12292.12 Da        Isoelectric Point: 5.8834

>AT191831 WP_063240604.1 NZ_CP069811:1780369-1780698 [Cupriavidus oxalaticus]
MARFENGMRPIHPGEILREEYLVPLNMSANALALALRVTPARISEIVREQRGITPDTALRLTRYFGGDARSWMNMQVTYD
LKVAQRDLGERIAAEVVPRAGIDQEIPES

Download         Length: 330 bp

>AT191831 NZ_CP091927:52019-52270 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAGCTGAAAAAAAATCCGATGGCAACTGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCAGCATTCTACTGTGTCCCTGCTGAGCTCTATGAAAGAATGC
TTGATGCCTTGGATGACCAGGAGCTGGTAAAACTGGTTACTGAACGCAGCAGTCAGCCTCTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A375GMD0

Antitoxin

Source ID Structure
AlphaFold DB A0A375GIG3

References