Detailed information of TA system TA191767

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 127406..127832
• Replicon: plasmid pTA13-1
• Accession: NZ_CP069713
• Organism: Escherichia coli strain EA13

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: JSQ77_RS23460
• Protein ID: WP_001312861.1
• Coordinates: 127406..127564 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	127608..127832 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSQ77_RS23425	122429..122656	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
JSQ77_RS23430	122793..123464	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
JSQ77_RS23435	123658..124041	-	384	WP_001354030.1	relaxosome protein TraM	-
JSQ77_RS23440	124364..124966	+	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
JSQ77_RS23445	125263..126084	-	822	WP_001234445.1	DUF945 domain-containing protein	-
JSQ77_RS23450	126195..126491	-	297	WP_001272251.1	hypothetical protein	-
JSQ77_RS23455	126791..127168	+	378	Protein_151	hypothetical protein	-
JSQ77_RS23460	127406..127564	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	127608..127832	-	225	NuclAT_0	-	Antitoxin
-	127608..127832	-	225	NuclAT_0	-	Antitoxin
-	127608..127832	-	225	NuclAT_0	-	Antitoxin
-	127608..127832	-	225	NuclAT_0	-	Antitoxin
JSQ77_RS23465	127644..127823	+	180	WP_001309233.1	hypothetical protein	-
JSQ77_RS23470	127844..128563	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
JSQ77_RS23475	128560..128994	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
JSQ77_RS23480	129063..131086	-	2024	Protein_156	ParB/RepB/Spo0J family partition protein	-
JSQ77_RS23485	131147..131380	-	234	WP_000006003.1	DUF905 family protein	-
JSQ77_RS23490	131438..131965	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / erm(42) / tet(A) / dfrA14 / mph(A) / aac(3)-IId / blaTEM-1B / sul3 / aph(3')-Ia / sitABCD	iutA / iucD / iucC / iucB / iucA	1..182585	182585

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T191767 WP_001312861.1 NZ_CP069713:c127564-127406 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T191767 NZ_CP091901:564151-564267 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGCTAGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTATCTTCGGTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT191767 NZ_CP069713:c127832-127608 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin