Detailed information of TA system TA191763

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3684042..3684736
• Replicon: chromosome
• Accession: NZ_CP069712
• Organism: Escherichia coli strain EA13

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: JSQ77_RS17730
• Protein ID: WP_112901532.1
• Coordinates: 3684042..3684440 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: JSQ77_RS17735
• Protein ID: WP_000554757.1
• Coordinates: 3684443..3684736 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSQ77_RS17700	3679042..3680286	-	1245	WP_000189541.1	esterase FrsA	-
-	3679702..3679782	-	81	NuclAT_9	-	-
-	3679702..3679782	-	81	NuclAT_9	-	-
-	3679702..3679782	-	81	NuclAT_9	-	-
-	3679702..3679782	-	81	NuclAT_9	-	-
JSQ77_RS17705	3680378..3680836	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JSQ77_RS17710	3681097..3682554	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
JSQ77_RS17715	3682611..3683132	-	522	Protein_3462	peptide chain release factor H	-
JSQ77_RS17720	3683131..3683334	-	204	Protein_3463	RNA ligase RtcB family protein	-
JSQ77_RS17725	3683580..3684032	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
JSQ77_RS17730	3684042..3684440	-	399	WP_112901532.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JSQ77_RS17735	3684443..3684736	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JSQ77_RS17740	3684788..3685843	-	1056	WP_001226164.1	DNA polymerase IV	-
JSQ77_RS17745	3685914..3686699	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
JSQ77_RS17750	3686671..3688383	+	1713	Protein_3469	flagellar biosynthesis protein FlhA	-
JSQ77_RS17755	3688607..3689104	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3683080..3705350	22270

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15502.85 Da
• Isoelectric Point: 7.4213

>T191763 WP_112901532.1 NZ_CP069712:c3684440-3684042 [Escherichia coli]
MWVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T191763 NZ_CP091899:2947671-2947814 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCGACTTTAATCTTCGGAATCCTAAAGGTTGTTAAAGAAGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT191763 WP_000554757.1 NZ_CP069712:c3684736-3684443 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT191763 NZ_CP091899:c2947560-2947418 [Enterococcus faecalis]
TTGTGCTATAATAGCAATGAAAAGAGAGATATGCTTCAACATACCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCTTT
TTAGTTACAAAAAATAACCGTACTCAGTCAAAGTTGACGGTTATTTTTTATTGTCATTTTTAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6