Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 702485..703212 Replicon chromosome
Accession NZ_CP069712
Organism Escherichia coli strain EA13

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag JSQ77_RS03385 Protein ID WP_000550189.1
Coordinates 702485..702799 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JSQ77_RS03390 Protein ID WP_000560266.1
Coordinates 702796..703212 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ77_RS03365 698652..699638 - 987 WP_112901449.1 Gfo/Idh/MocA family oxidoreductase -
JSQ77_RS03370 699717..700400 - 684 WP_001183042.1 vancomycin high temperature exclusion protein -
JSQ77_RS03375 700477..700980 - 504 WP_001295542.1 M48 family metallopeptidase -
JSQ77_RS03380 701065..702201 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
JSQ77_RS03385 702485..702799 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
JSQ77_RS03390 702796..703212 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
JSQ77_RS03395 703257..705275 - 2019 WP_023281493.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
JSQ77_RS03400 705701..708052 - 2352 WP_024238710.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T191744 WP_000550189.1 NZ_CP069712:702485-702799 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T191744 NZ_CP091895:c2639640-2639539 [Enterococcus faecalis]
TTGTCAATCGAAGCGACATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCACTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACGATAAAAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT191744 WP_000560266.1 NZ_CP069712:702796-703212 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT191744 NZ_CP091895:2639416-2639594 [Enterococcus faecalis]
AAAAGAGAGGTATGCGGGTACATACCTCTCCTTTTATACCAACACCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGTT
TTTGTTGTTCTTTAACCGTTCAGCCGTCTAAGCTTATGAACGGTTATTTTTTATCGTTTTTCGTTGCTTCAAGGATACCG
AAAATCAGTAGTGCAACAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References