Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 659162..659961 Replicon chromosome
Accession NZ_CP069712
Organism Escherichia coli strain EA13

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag JSQ77_RS03165 Protein ID WP_000347273.1
Coordinates 659162..659626 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JSQ77_RS03170 Protein ID WP_001307405.1
Coordinates 659626..659961 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ77_RS03135 654163..654597 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
JSQ77_RS03140 654615..655493 - 879 WP_001300474.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
JSQ77_RS03145 655483..656262 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
JSQ77_RS03150 656273..656746 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JSQ77_RS03155 656769..658049 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
JSQ77_RS03160 658298..659107 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JSQ77_RS03165 659162..659626 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JSQ77_RS03170 659626..659961 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JSQ77_RS03175 660110..661681 - 1572 WP_001723936.1 galactarate dehydratase -
JSQ77_RS03180 662056..663390 + 1335 WP_089075269.1 galactarate/glucarate/glycerate transporter GarP -
JSQ77_RS03185 663406..664176 + 771 WP_001723934.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T191743 WP_000347273.1 NZ_CP069712:c659626-659162 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T191743 NZ_CP091895:1805745-1806089 [Enterococcus faecalis]
ATGAAAAGTATCAAAGAGTTGGTGGAAGAATATGAAGTAGAGTTAGTTTTCGCTCCAATAAATAAGCGCGCATGTTACGA
GCCAGTCAAAAGAATAATTTTCGTAAATCAAAATTTATCTATCGAAGAACAAGAAGAGTCTATATTCCATGAGTTCAAAC
ATGTTGTCTCCCATTCGGATTATATTGAGTTATATAAAATTCCTTCTTTTAGAAATAAGATGGAAGCTGAGGCAGATTAT
CATATGTTTAAATGTCTTATTGAAAAACATGACGGGCAATTTAATTATTCTAATGTGATTACTCATTACAATTTAAAGAT
GGGACAGGAAACTTATTTAAATTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT191743 WP_001307405.1 NZ_CP069712:c659961-659626 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT191743 NZ_CP091895:1805396-1805728 [Enterococcus faecalis]
ATGAACCTCTTGGAACGTATAAAAAAACTAGCAAAAGATAGAGATGTGTCTATTTATCAGCTTGAAGAAGAAATAAATAT
TGGACGAAATACTATTTATCAATGGAATAAGCGAACACCTTCTTCGGATAAACTTGAAGCTGTAGCCAATTATTTTAATG
TTTCTGTTGATTATTTATTAGGTCGTACTGAAAATCCAAACCAAGCTGGTGCTAAACCATCTGATGATTTAGATGAAGTA
TTAGACAATGTGATGAGTTTCGATGGTGAGCCTTTGGATGATCATGATCGGGAAGTTATTCGTGCTTATTTGAAAGGGAG
ATTCGGAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References