Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3629686..3630227 Replicon chromosome
Accession NZ_CP069709
Organism Escherichia coli strain EA7

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag JSQ43_RS18085 Protein ID WP_000615979.1
Coordinates 3629949..3630227 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag JSQ43_RS18080 Protein ID WP_000729704.1
Coordinates 3629686..3629946 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ43_RS18060 3625369..3626154 - 786 WP_204147906.1 putative lateral flagellar export/assembly protein LafU -
JSQ43_RS18065 3626126..3627838 + 1713 Protein_3443 flagellar biosynthesis protein FlhA -
JSQ43_RS18070 3628054..3628551 - 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
JSQ43_RS18075 3628727..3629500 - 774 WP_001353765.1 C40 family peptidase -
JSQ43_RS18080 3629686..3629946 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JSQ43_RS18085 3629949..3630227 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JSQ43_RS18090 3630383..3631123 + 741 WP_001225679.1 murein L,D-transpeptidase -
JSQ43_RS18095 3631094..3631861 - 768 WP_000333379.1 class II glutamine amidotransferase -
JSQ43_RS18100 3632067..3632645 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T191716 WP_000615979.1 NZ_CP069709:3629949-3630227 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T191716 NZ_CP091889:c2916903-2916802 [Enterococcus faecalis]
TTGTCAATCGAAGCGGCATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCACTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACGATAAAAAATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT191716 WP_000729704.1 NZ_CP069709:3629686-3629946 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT191716 NZ_CP091889:2916678-2916857 [Enterococcus faecalis]
TGAAAAGAGAGATATGCTACTACATACCTCTCCTTTATACCAACACCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGT
TTTTGTTGTTCTTTAACCGTTCAGCCGTCTAAGCTTATGAACGGTTATTTTTTATCGTTTTTCGTTGCTTCAAGGATACC
GAAAATCAGTAGTGCAACAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References