Detailed information of TA system TA191715

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3623497..3624191
• Replicon: chromosome
• Accession: NZ_CP069709
• Organism: Escherichia coli strain EA7

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: JSQ43_RS18045
• Protein ID: WP_001263493.1
• Coordinates: 3623497..3623895 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: JSQ43_RS18050
• Protein ID: WP_000554757.1
• Coordinates: 3623898..3624191 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSQ43_RS18015	3618497..3619741	-	1245	WP_000189541.1	esterase FrsA	-
-	3619157..3619237	-	81	NuclAT_9	-	-
-	3619157..3619237	-	81	NuclAT_9	-	-
-	3619157..3619237	-	81	NuclAT_9	-	-
-	3619157..3619237	-	81	NuclAT_9	-	-
JSQ43_RS18020	3619833..3620291	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JSQ43_RS18025	3620552..3622009	+	1458	WP_001292997.1	cytosol nonspecific dipeptidase	-
JSQ43_RS18030	3622066..3622587	-	522	Protein_3436	peptide chain release factor H	-
JSQ43_RS18035	3622586..3622789	-	204	Protein_3437	RNA ligase RtcB family protein	-
JSQ43_RS18040	3623035..3623487	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
JSQ43_RS18045	3623497..3623895	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JSQ43_RS18050	3623898..3624191	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JSQ43_RS18055	3624243..3625298	-	1056	WP_001226164.1	DNA polymerase IV	-
JSQ43_RS18060	3625369..3626154	-	786	WP_204147906.1	putative lateral flagellar export/assembly protein LafU	-
JSQ43_RS18065	3626126..3627838	+	1713	Protein_3443	flagellar biosynthesis protein FlhA	-
JSQ43_RS18070	3628054..3628551	-	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T191715 WP_001263493.1 NZ_CP069709:c3623895-3623497 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T191715 NZ_CP091889:c2916470-2916369 [Enterococcus faecalis]
TTGTCAATCGAAGCGGCATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCACTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACGATAAAAAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT191715 WP_000554757.1 NZ_CP069709:c3624191-3623898 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT191715 NZ_CP091889:2916247-2916424 [Enterococcus faecalis]
AAAAGAGAGGTATGCGGGTACATACCTCTCTTTTATACCAACACCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGTTT
TTATTACTGTTTAACCGTTCGGCCTGTCAAGCTTATGAACGGTTATTTTTTATCGTTTTTCGTTGCTTCAAGGATACCGA
AAATCAGTAGTGCAACAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6