Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1753450..1753952 Replicon chromosome
Accession NZ_CP069709
Organism Escherichia coli strain EA7

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag JSQ43_RS08715 Protein ID WP_000767829.1
Coordinates 1753698..1753952 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag JSQ43_RS08710 Protein ID WP_001259255.1
Coordinates 1753450..1753701 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ43_RS08685 1748628..1749695 - 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
JSQ43_RS08690 1749695..1750765 - 1071 WP_000108967.1 histidinol-phosphate transaminase -
JSQ43_RS08695 1750762..1752066 - 1305 WP_001593467.1 histidinol dehydrogenase -
JSQ43_RS08700 1752072..1752971 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
JSQ43_RS08705 1753117..1753167 - 51 WP_001364200.1 his operon leader peptide -
JSQ43_RS08710 1753450..1753701 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
JSQ43_RS08715 1753698..1753952 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
JSQ43_RS08720 1754035..1754859 + 825 WP_000754758.1 SDR family oxidoreductase -
JSQ43_RS08725 1754905..1755834 + 930 WP_000803370.1 LysR family transcriptional regulator -
JSQ43_RS08730 1756049..1756111 + 63 WP_010723108.1 membrane protein YoeI -
JSQ43_RS08735 1756101..1757459 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
JSQ43_RS08740 1757638..1758696 + 1059 WP_000492346.1 transport protein YeeE -
JSQ43_RS08745 1758710..1758937 + 228 WP_000985270.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T191703 WP_000767829.1 NZ_CP069709:1753698-1753952 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T191703 NZ_CP091886:c334828-334733 [Enterococcus faecalis]
ATGTACGAGATTGTCACAAAAATTCTTGTGCCGATTTTTGTCGGGATTGTCCTGAAACTTGTAACCATTTGGTTGGAAAA
ACAGAACGAGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT191703 WP_001259255.1 NZ_CP069709:1753450-1753701 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT191703 NZ_CP091886:334633-334698 [Enterococcus faecalis]
CTTGTGACAAAGTCGTGCATGGATGCACTAAAAAGACACCCTATCAGTAGTAGGGTGTCTTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References