Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 638540..639267 Replicon chromosome
Accession NZ_CP069709
Organism Escherichia coli strain EA7

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag JSQ43_RS03520 Protein ID WP_000550189.1
Coordinates 638540..638854 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JSQ43_RS03525 Protein ID WP_059328945.1
Coordinates 638851..639267 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ43_RS03500 634698..635684 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
JSQ43_RS03505 635763..636455 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
JSQ43_RS03510 636532..637035 - 504 WP_001300832.1 M48 family metallopeptidase -
JSQ43_RS03515 637120..638256 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
JSQ43_RS03520 638540..638854 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
JSQ43_RS03525 638851..639267 + 417 WP_059328945.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
JSQ43_RS03530 639312..641330 - 2019 WP_112958558.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
JSQ43_RS03535 641756..644107 - 2352 WP_000695489.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-136)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T191699 WP_000550189.1 NZ_CP069709:638540-638854 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T191699 NZ_CP091884:c2848111-2847770 [Enterococcus faecalis]
ATGAATGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15094.55 Da        Isoelectric Point: 4.5822

>AT191699 WP_059328945.1 NZ_CP069709:638851-639267 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSRKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT191699 NZ_CP091884:c2848341-2848111 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References