Detailed information of TA system TA191696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3832809..3833503
• Replicon: chromosome
• Accession: NZ_CP069707
• Organism: Escherichia coli strain E41

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: JSQ86_RS18970
• Protein ID: WP_001263493.1
• Coordinates: 3832809..3833207 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: JSQ86_RS18975
• Protein ID: WP_000554757.1
• Coordinates: 3833210..3833503 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSQ86_RS18940	3827809..3829053	-	1245	WP_000189541.1	esterase FrsA	-
-	3828469..3828549	-	81	NuclAT_11	-	-
-	3828469..3828549	-	81	NuclAT_11	-	-
-	3828469..3828549	-	81	NuclAT_11	-	-
-	3828469..3828549	-	81	NuclAT_11	-	-
JSQ86_RS18945	3829145..3829603	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JSQ86_RS18950	3829864..3831321	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
JSQ86_RS18955	3831378..3831899	-	522	Protein_3714	peptide chain release factor H	-
JSQ86_RS18960	3831898..3832101	-	204	Protein_3715	RNA ligase RtcB family protein	-
JSQ86_RS18965	3832347..3832799	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
JSQ86_RS18970	3832809..3833207	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JSQ86_RS18975	3833210..3833503	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JSQ86_RS18980	3833555..3834610	-	1056	WP_001226164.1	DNA polymerase IV	-
JSQ86_RS18985	3834681..3835604	-	924	WP_001232547.1	putative lateral flagellar export/assembly protein LafU	-
JSQ86_RS18990	3835607..3836470	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
JSQ86_RS18995	3836483..3837199	-	717	WP_000938730.1	FliA/WhiG family RNA polymerase sigma factor	-
JSQ86_RS19000	3837219..3837686	-	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T191696 WP_001263493.1 NZ_CP069707:c3833207-3832809 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T191696 NZ_CP091884:628292-628408 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGCTAGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTATCTTCGGTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT191696 WP_000554757.1 NZ_CP069707:c3833503-3833210 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT191696 NZ_CP091884:c628524-628319 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTTTCTAATAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6