Detailed information of TA system TA191673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 3716976..3717517
• Replicon: chromosome
• Accession: NZ_CP069706
• Organism: Escherichia coli strain ECY44

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: E9TC49
• Locus tag: JR341_RS21050
• Protein ID: WP_000615982.1
• Coordinates: 3717239..3717517 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: S1EWQ0
• Locus tag: JR341_RS21045
• Protein ID: WP_000729704.1
• Coordinates: 3716976..3717236 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JR341_RS21020	3712012..3713151	+	1140	WP_000785664.1	flagellar type III secretion system protein FlhB	-
JR341_RS21025	3713135..3715228	+	2094	WP_001583165.1	flagellar biosynthesis protein FlhA	-
JR341_RS21030	3715333..3715611	+	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
JR341_RS21035	3715604..3715960	+	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-
JR341_RS21040	3716017..3716790	-	774	WP_000543899.1	C40 family peptidase	-
JR341_RS21045	3716976..3717236	+	261	WP_000729704.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
JR341_RS21050	3717239..3717517	+	279	WP_000615982.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
JR341_RS21055	3717673..3718413	+	741	WP_001225679.1	murein L,D-transpeptidase	-
JR341_RS21060	3718384..3719151	-	768	WP_000333380.1	class II glutamine amidotransferase	-
JR341_RS21065	3719357..3719935	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-84)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10816.59 Da
• Isoelectric Point: 10.0702

>T191673 WP_000615982.1 NZ_CP069706:3717239-3717517 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T191673 NZ_CP091878:226347-226832 [Salmonella enterica subsp. enterica serovar Tennessee]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTTGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGGGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9391.77 Da
• Isoelectric Point: 5.0758

>AT191673 WP_000729704.1 NZ_CP069706:3716976-3717236 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT191673 NZ_CP091878:226072-226359 [Salmonella enterica subsp. enterica serovar Tennessee]
ATGCTATACAAAGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCGGGCTGCCGAGAATGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6Z6