191656

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 585821..586620 Replicon chromosome
Accession NZ_CP069706
Organism Escherichia coli strain ECY44

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag JR341_RS05740 Protein ID WP_000347267.1
Coordinates 585821..586285 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JR341_RS05745 Protein ID WP_001307405.1
Coordinates 586285..586620 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JR341_RS05710 580822..581256 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
JR341_RS05715 581274..582152 - 879 WP_001298758.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
JR341_RS05720 582142..582921 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
JR341_RS05725 582932..583405 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
JR341_RS05730 583428..584708 - 1281 WP_000681947.1 tagatose-bisphosphate aldolase subunit KbaZ -
JR341_RS05735 584957..585766 + 810 WP_000072180.1 aga operon transcriptional regulator AgaR -
JR341_RS05740 585821..586285 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JR341_RS05745 586285..586620 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JR341_RS05750 586769..588340 - 1572 WP_001273741.1 galactarate dehydratase -
JR341_RS05755 588715..590049 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
JR341_RS05760 590065..590835 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 585821..597276 11455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T191656 WP_000347267.1 NZ_CP069706:c586285-585821 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T191656 NZ_CP091875:c2196576-2196325 [Staphylococcus aureus]
ATGAATAATCGCGAACAAATTGAACAATCAGTTATAAGTGCTAGTGCGTATAACGGTAATGACACAGAGGGATTACTAAA
AGAGATTGAGGACGTTTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGAGGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTGTTGAACTTGATGAAGCAGTAGGGATTATGGCAGGTCAAGTTGTCTATAAATATGAGGAGGAACAGGAA
AATGAGCATTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT191656 WP_001307405.1 NZ_CP069706:c586620-586285 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT191656 NZ_CP091875:c2196335-2196153 [Staphylococcus aureus]
ATGAGCATTAGTGTAGGAGACAAGGTTTTTAATCCAGAAACAAATTCAACTTTAGAAATTGTACAACTTGTTGGCGATAT
TAGAGACACGCATTACAAGTTATCTGACGGATCTATTATTAGTCTTATAGACTTTGTTGTTAAACCAATTCATTTAATCA
AGGAGGAGCAGGAAAATGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References