Detailed information of TA system TA191648

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 88000..88264
• Replicon: plasmid pECY44-2
• Accession: NZ_CP069703
• Organism: Escherichia coli strain ECY44

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: JR341_RS01745
• Protein ID: WP_001331364.1
• Coordinates: 88112..88264 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	88000..88062 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JR341_RS01730	83239..85530	-	2292	WP_001289263.1	hypothetical protein	-
JR341_RS01735	85523..86593	-	1071	WP_072645269.1	IncI1-type conjugal transfer protein TrbB	-
JR341_RS01740	86612..87820	-	1209	WP_072645270.1	IncI1-type conjugal transfer protein TrbA	-
-	88000..88062	-	63	NuclAT_1	-	Antitoxin
-	88000..88062	-	63	NuclAT_1	-	Antitoxin
-	88000..88062	-	63	NuclAT_1	-	Antitoxin
-	88000..88062	-	63	NuclAT_1	-	Antitoxin
JR341_RS01745	88112..88264	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
JR341_RS01750	88336..88587	-	252	WP_021513960.1	hypothetical protein	-
JR341_RS01755	88803..89972	+	1170	WP_072645271.1	transposase	-
-	90312..90363	-	52	NuclAT_2	-	-
-	90312..90363	-	52	NuclAT_2	-	-
-	90312..90363	-	52	NuclAT_2	-	-
-	90312..90363	-	52	NuclAT_2	-	-
JR341_RS01760	91203..91379	-	177	WP_001054905.1	hypothetical protein	-
JR341_RS01765	91588..91797	-	210	WP_001140544.1	hemolysin expression modulator Hha	-
JR341_RS01770	91895..92557	-	663	WP_072645273.1	plasmid IncI1-type surface exclusion protein ExcA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..135712	135712
	-	flank	IS/Tn	-	-	88803..89972	1169

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T191648 WP_001331364.1 NZ_CP069703:88112-88264 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T191648 NZ_CP091872:2570772-2570951 [Bacillus sp. Man122]
ATGTCGACCTATGAATCTCTAATGGTCATGATCGGCTTTGCCAATTTAATAGGCGGGATTATGACATGGGTAATATCTCT
TTTAACATTATTATTCATGCTTAGAAAAAAAGACACTCATCCTATTTACATTACTGTAAAGGAAAAGTGTCTACACGAGG
ACCCTCCTATTAAAGGGTAG

Antitoxin

Nucleotide

• Length: 63 bp

>AT191648 NZ_CP069703:c88062-88000 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin