Detailed information of TA system TA191638

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 42849..43088
• Replicon: plasmid pMUB-MIN6-1
• Accession: NZ_CP069693
• Organism: Escherichia coli H20 strain MIN6

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: JSU10_RS24215
• Protein ID: WP_023144756.1
• Coordinates: 42849..42983 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	43028..43088 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSU10_RS24185 (38550)	38550..39407	-	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-
JSU10_RS24190 (39400)	39400..39474	-	75	WP_031943482.1	RepA leader peptide Tap	-
JSU10_RS24200 (39836)	39836..41377	+	1542	WP_002431311.1	IS21-like element ISEc12 family transposase	-
JSU10_RS24205 (41392)	41392..42138	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
JSU10_RS24210 (42298)	42298..42552	-	255	WP_000083850.1	replication regulatory protein RepA	-
JSU10_RS24215 (42849)	42849..42983	-	135	WP_023144756.1	Hok/Gef family protein	Toxin
- (43028)	43028..43088	+	61	NuclAT_0	-	Antitoxin
- (43028)	43028..43088	+	61	NuclAT_0	-	Antitoxin
- (43028)	43028..43088	+	61	NuclAT_0	-	Antitoxin
- (43028)	43028..43088	+	61	NuclAT_0	-	Antitoxin
JSU10_RS24220 (43055)	43055..43341	-	287	Protein_60	DUF2726 domain-containing protein	-
JSU10_RS24225 (43854)	43854..44066	-	213	WP_013023861.1	hypothetical protein	-
JSU10_RS24235 (44914)	44914..45297	-	384	Protein_63	DUF932 domain-containing protein	-
JSU10_RS24240 (45416)	45416..45703	-	288	WP_000107535.1	hypothetical protein	-
JSU10_RS24245 (45728)	45728..45934	-	207	WP_000275859.1	hypothetical protein	-
JSU10_RS25510 (46175)	46175..46438	-	264	WP_071781663.1	hypothetical protein	-
JSU10_RS24250 (46345)	46345..46563	-	219	WP_001496291.1	hypothetical protein	-
JSU10_RS24255 (46589)	46589..47152	-	564	WP_001348621.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-Ia / blaTEM-1B / dfrA1 / ant(3'')-Ia / qacE / sul1 / aph(6)-Id / aph(3'')-Ib / sul2 / tet(B) / sitABCD	iutA / iucD / iucC / iucB / iucA	1..89956	89956
	-	inside	IScluster/Tn	-	-	39836..44858	5022

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T191638 WP_023144756.1 NZ_CP069693:c42983-42849 [Escherichia coli H20]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T191638 NZ_CP091855:c1696452-1696162 [Gordonia amicalis]
ATGAGCGACGCTCCGACAGACTCGCCCGCCCCGTACCGGGTCGAGGTCGCATCCCCAGCTCGCCGCGACCTTCAACGCCT
TCCATCCCGGATCGTCCACGCCGTCATCGAATTCATCTCCGGACCTCTCGCTGGTAACCCGCACCGGCTCAGCAAGCCGC
TGCGCAACGACCTCGCCGGCCTCCACAGCGCCCGACGCGGCGACTACCGCGTCCTGCTCCGCATCGACGACCCGAACCAC
ACCATCGTTGTCGTCAGAATCGACCACCGTGCCCACGCCTATCGCACCTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT191638 NZ_CP069693:43028-43088 [Escherichia coli H20]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin