Detailed information of TA system TA191637

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4786780..4787382
• Replicon: chromosome
• Accession: NZ_CP069692
• Organism: Escherichia coli H20 strain MIN6

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JSU10_RS22940
• Protein ID: WP_000897305.1
• Coordinates: 4787071..4787382 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JSU10_RS22935
• Protein ID: WP_000356397.1
• Coordinates: 4786780..4787070 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSU10_RS22910 (4782706)	4782706..4783608	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JSU10_RS22915 (4783605)	4783605..4784240	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JSU10_RS22920 (4784237)	4784237..4785166	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
JSU10_RS22925 (4785496)	4785496..4785738	-	243	WP_001086388.1	protein YiiF	-
JSU10_RS22930 (4785957)	4785957..4786175	-	219	WP_001297075.1	CopG family transcriptional regulator	-
JSU10_RS22935 (4786780)	4786780..4787070	-	291	WP_000356397.1	NadS family protein	Antitoxin
JSU10_RS22940 (4787071)	4787071..4787382	-	312	WP_000897305.1	hypothetical protein	Toxin
JSU10_RS22945 (4787611)	4787611..4788519	+	909	WP_001162704.1	alpha/beta hydrolase	-
JSU10_RS22950 (4788583)	4788583..4789524	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JSU10_RS22955 (4789569)	4789569..4790006	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
JSU10_RS22960 (4790003)	4790003..4790875	-	873	WP_000920762.1	virulence factor BrkB family protein	-
JSU10_RS22965 (4790869)	4790869..4791468	-	600	WP_001295269.1	glucose-1-phosphatase	-
JSU10_RS22970 (4791567)	4791567..4792352	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T191637 WP_000897305.1 NZ_CP069692:c4787382-4787071 [Escherichia coli H20]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T191637 NZ_CP091848:55121-55603 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT191637 WP_000356397.1 NZ_CP069692:c4787070-4786780 [Escherichia coli H20]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT191637 NZ_CP091848:54867-55133 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7