191632

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3871077..3871618 Replicon chromosome
Accession NZ_CP069692
Organism Escherichia coli H20 strain MIN6

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag JSU10_RS18725 Protein ID WP_000615976.1
Coordinates 3871340..3871618 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag JSU10_RS18720 Protein ID WP_000729704.1
Coordinates 3871077..3871337 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU10_RS18695 (3866860) 3866860..3867645 - 786 WP_000207589.1 putative lateral flagellar export/assembly protein LafU -
JSU10_RS18700 (3867617) 3867617..3869329 + 1713 Protein_3713 flagellar biosynthesis protein FlhA -
JSU10_RS18705 (3869434) 3869434..3869712 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
JSU10_RS18710 (3869705) 3869705..3870061 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
JSU10_RS18715 (3870118) 3870118..3870891 - 774 WP_001396221.1 C40 family peptidase -
JSU10_RS18720 (3871077) 3871077..3871337 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JSU10_RS18725 (3871340) 3871340..3871618 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JSU10_RS18730 (3871774) 3871774..3872514 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
JSU10_RS18735 (3872485) 3872485..3873252 - 768 WP_000333380.1 class II glutamine amidotransferase -
JSU10_RS18740 (3873458) 3873458..3874036 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T191632 WP_000615976.1 NZ_CP069692:3871340-3871618 [Escherichia coli H20]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T191632 NZ_CP091846:c4645363-4644830 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT191632 WP_000729704.1 NZ_CP069692:3871077-3871337 [Escherichia coli H20]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT191632 NZ_CP091846:c4645640-4645374 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References