Detailed information of TA system TA191628

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralR-agrB/-
• Location: 2688594..2688953
• Replicon: chromosome
• Accession: NZ_CP069692
• Organism: Escherichia coli H20 strain MIN6

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: JSU10_RS12885
• Protein ID: WP_001317028.1
• Coordinates: 2688594..2688788 (+)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	2688787..2688953 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSU10_RS12860 (2684292)	2684292..2684513	+	222	WP_000560224.1	killing protein KilR	-
JSU10_RS12865 (2684513)	2684513..2684683	+	171	WP_001352098.1	YdaE family protein	-
JSU10_RS12870 (2684758)	2684758..2685033	+	276	WP_024251429.1	hypothetical protein	-
JSU10_RS12875 (2685135)	2685135..2687735	+	2601	WP_000105136.1	exodeoxyribonuclease VIII	-
JSU10_RS12880 (2687728)	2687728..2688537	+	810	WP_000166319.1	recombination protein RecT	-
JSU10_RS12885 (2688594)	2688594..2688788	+	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
- (2688787)	2688787..2688953	-	167	NuclAT_1	-	Antitoxin
- (2688787)	2688787..2688953	-	167	NuclAT_1	-	Antitoxin
- (2688787)	2688787..2688953	-	167	NuclAT_1	-	Antitoxin
- (2688787)	2688787..2688953	-	167	NuclAT_1	-	Antitoxin
JSU10_RS12890 (2688781)	2688781..2688978	+	198	WP_024170988.1	DUF1187 family protein	-
JSU10_RS12895 (2689057)	2689057..2689272	+	216	WP_000079604.1	excisionase XisR	-
JSU10_RS12900 (2689274)	2689274..2690509	+	1236	WP_000040852.1	site-specific integrase	-
JSU10_RS12905 (2690561)	2690561..2691496	+	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
JSU10_RS12910 (2691625)	2691625..2692998	-	1374	WP_000123739.1	ATP-dependent RNA helicase DbpA	-
JSU10_RS12915 (2693028)	2693028..2693201	-	174	WP_001296046.1	protein YnaL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2639699..2690509	50810

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T191628 WP_001317028.1 NZ_CP069692:2688594-2688788 [Escherichia coli H20]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T191628 NZ_CP091846:738908-739393 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Nucleotide

• Length: 167 bp

>AT191628 NZ_CP069692:c2688953-2688787 [Escherichia coli H20]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAATTTTACCTGAGAGCATTTTTTC
GCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTTCAATAGTGGCGG
TAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin