Detailed information of TA system TA191600

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 81811..82065
• Replicon: plasmid pMUB-MIN10-1
• Accession: NZ_CP069678
• Organism: Escherichia coli O126:H45 strain MIN10

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: JSU12_RS25310
• Protein ID: WP_001312851.1
• Coordinates: 81811..81960 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	82004..82065 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSU12_RS25265 (77051)	77051..77203	+	153	Protein_76	IS4 family transposase	-
JSU12_RS25270 (77363)	77363..77764	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
JSU12_RS25275 (77697)	77697..77954	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
JSU12_RS25280 (78047)	78047..78700	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
JSU12_RS25285 (78798)	78798..78938	-	141	WP_001333237.1	hypothetical protein	-
JSU12_RS25290 (79639)	79639..80496	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
JSU12_RS25295 (80489)	80489..80971	-	483	WP_001273588.1	hypothetical protein	-
JSU12_RS26835 (80964)	80964..81011	-	48	WP_229471593.1	hypothetical protein	-
JSU12_RS26840 (81002)	81002..81253	+	252	WP_223195197.1	replication protein RepA	-
JSU12_RS25305 (81270)	81270..81527	-	258	WP_000083845.1	replication regulatory protein RepA	-
JSU12_RS25310 (81811)	81811..81960	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (82004)	82004..82065	+	62	NuclAT_1	-	Antitoxin
- (82004)	82004..82065	+	62	NuclAT_1	-	Antitoxin
- (82004)	82004..82065	+	62	NuclAT_1	-	Antitoxin
- (82004)	82004..82065	+	62	NuclAT_1	-	Antitoxin
JSU12_RS25315 (82321)	82321..82395	-	75	Protein_87	endonuclease	-
JSU12_RS25320 (82641)	82641..82853	-	213	WP_001299730.1	ANR family transcriptional regulator	-
JSU12_RS25325 (82989)	82989..83549	-	561	WP_000139315.1	fertility inhibition protein FinO	-
JSU12_RS25330 (83652)	83652..84512	-	861	WP_000704514.1	alpha/beta hydrolase	-
JSU12_RS25335 (84571)	84571..85317	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / sul3 / ant(3'')-Ia / dfrA1 / tet(A) / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..146908	146908

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T191600 WP_001312851.1 NZ_CP069678:c81960-81811 [Escherichia coli O126:H45]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T191600 NZ_CP091836:3453685-3453858 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT191600 NZ_CP069678:82004-82065 [Escherichia coli O126:H45]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin