Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4881288..4881890 Replicon chromosome
Accession NZ_CP069677
Organism Escherichia coli O126:H45 strain MIN10

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag JSU12_RS23660 Protein ID WP_000897305.1
Coordinates 4881579..4881890 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JSU12_RS23655 Protein ID WP_000356397.1
Coordinates 4881288..4881578 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU12_RS23630 (4877214) 4877214..4878116 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
JSU12_RS23635 (4878113) 4878113..4878748 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
JSU12_RS23640 (4878745) 4878745..4879674 + 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
JSU12_RS23645 (4880004) 4880004..4880246 - 243 WP_001086388.1 protein YiiF -
JSU12_RS23650 (4880465) 4880465..4880683 - 219 WP_001295676.1 CopG family transcriptional regulator -
JSU12_RS23655 (4881288) 4881288..4881578 - 291 WP_000356397.1 NadS family protein Antitoxin
JSU12_RS23660 (4881579) 4881579..4881890 - 312 WP_000897305.1 hypothetical protein Toxin
JSU12_RS23665 (4882119) 4882119..4883027 + 909 WP_001162704.1 alpha/beta hydrolase -
JSU12_RS23670 (4883091) 4883091..4884032 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
JSU12_RS23675 (4884077) 4884077..4884514 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
JSU12_RS23680 (4884511) 4884511..4885383 - 873 WP_000920762.1 virulence factor BrkB family protein -
JSU12_RS23685 (4885377) 4885377..4885976 - 600 WP_001295269.1 glucose-1-phosphatase -
JSU12_RS23690 (4886075) 4886075..4886860 - 786 WP_000059678.1 DeoR/GlpR family DNA-binding transcription regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T191599 WP_000897305.1 NZ_CP069677:c4881890-4881579 [Escherichia coli O126:H45]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T191599 NZ_CP091836:c2767518-2767411 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT191599 WP_000356397.1 NZ_CP069677:c4881578-4881288 [Escherichia coli O126:H45]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT191599 NZ_CP091836:2767566-2767632 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References