Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4027372..4027913 Replicon chromosome
Accession NZ_CP069677
Organism Escherichia coli O126:H45 strain MIN10

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag JSU12_RS19740 Protein ID WP_000615976.1
Coordinates 4027635..4027913 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag JSU12_RS19735 Protein ID WP_000729704.1
Coordinates 4027372..4027632 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU12_RS19710 (4023155) 4023155..4023940 - 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -
JSU12_RS19715 (4023912) 4023912..4025624 + 1713 Protein_3897 flagellar biosynthesis protein FlhA -
JSU12_RS19720 (4025729) 4025729..4026007 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
JSU12_RS19725 (4026000) 4026000..4026356 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
JSU12_RS19730 (4026413) 4026413..4027186 - 774 WP_000543897.1 C40 family peptidase -
JSU12_RS19735 (4027372) 4027372..4027632 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JSU12_RS19740 (4027635) 4027635..4027913 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JSU12_RS19745 (4028069) 4028069..4028809 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
JSU12_RS19750 (4028780) 4028780..4029547 - 768 WP_000333380.1 class II glutamine amidotransferase -
JSU12_RS19755 (4029753) 4029753..4030331 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T191595 WP_000615976.1 NZ_CP069677:4027635-4027913 [Escherichia coli O126:H45]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T191595 NZ_CP091836:c2100987-2100580 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT191595 WP_000729704.1 NZ_CP069677:4027372-4027632 [Escherichia coli O126:H45]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT191595 NZ_CP091836:c2101234-2100968 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References