Detailed information of TA system TA191577

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 596064..596863
• Replicon: chromosome
• Accession: NZ_CP069677
• Organism: Escherichia coli O126:H45 strain MIN10

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: U9XVR9
• Locus tag: JSU12_RS02895
• Protein ID: WP_000347267.1
• Coordinates: 596064..596528 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: JSU12_RS02900
• Protein ID: WP_001307405.1
• Coordinates: 596528..596863 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSU12_RS02865 (591065)	591065..591499	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
JSU12_RS02870 (591517)	591517..592395	-	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
JSU12_RS02875 (592385)	592385..593164	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
JSU12_RS02880 (593175)	593175..593648	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
JSU12_RS02885 (593671)	593671..594951	-	1281	WP_000681936.1	tagatose-bisphosphate aldolase subunit KbaZ	-
JSU12_RS02890 (595200)	595200..596009	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
JSU12_RS02895 (596064)	596064..596528	-	465	WP_000347267.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
JSU12_RS02900 (596528)	596528..596863	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
JSU12_RS02905 (597012)	597012..598583	-	1572	WP_001273741.1	galactarate dehydratase	-
JSU12_RS02910 (598958)	598958..600292	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
JSU12_RS02915 (600308)	600308..601078	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17807.17 Da
• Isoelectric Point: 9.4947

>T191577 WP_000347267.1 NZ_CP069677:c596528-596064 [Escherichia coli O126:H45]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T191577 NZ_CP091813:c5237063-5236680 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCCTCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT191577 WP_001307405.1 NZ_CP069677:c596863-596528 [Escherichia coli O126:H45]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT191577 NZ_CP091813:c5237305-5237063 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A0E0XTR4

Antitoxin

• AlphaFold DB: A0A0E0XVC7