Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3582473..3583014 Replicon chromosome
Accession NZ_CP069666
Organism Escherichia coli O89m:H9 strain MIN11

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag JSU13_RS17185 Protein ID WP_000615983.1
Coordinates 3582736..3583014 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag JSU13_RS17180 Protein ID WP_000729703.1
Coordinates 3582473..3582733 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU13_RS17160 (3578148) 3578148..3578933 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
JSU13_RS17165 (3578905) 3578905..3580617 + 1713 Protein_3400 flagellar biosynthesis protein FlhA -
JSU13_RS17170 (3580841) 3580841..3581338 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
JSU13_RS17175 (3581514) 3581514..3582263 - 750 WP_000056849.1 C40 family peptidase -
JSU13_RS17180 (3582473) 3582473..3582733 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JSU13_RS17185 (3582736) 3582736..3583014 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JSU13_RS17190 (3583170) 3583170..3583910 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
JSU13_RS17195 (3583881) 3583881..3584648 - 768 WP_000333380.1 class II glutamine amidotransferase -
JSU13_RS17200 (3584854) 3584854..3585432 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T191567 WP_000615983.1 NZ_CP069666:3582736-3583014 [Escherichia coli O89m:H9]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T191567 NZ_CP091806:c80161-79679 [Klebsiella quasipneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGCAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT191567 WP_000729703.1 NZ_CP069666:3582473-3582733 [Escherichia coli O89m:H9]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT191567 NZ_CP091806:c80415-80149 [Klebsiella quasipneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References