Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 3576276..3576970 | Replicon | chromosome |
| Accession | NZ_CP069666 | ||
| Organism | Escherichia coli O89m:H9 strain MIN11 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | Q47157 |
| Locus tag | JSU13_RS17145 | Protein ID | WP_001263489.1 |
| Coordinates | 3576276..3576674 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | S1QAE3 |
| Locus tag | JSU13_RS17150 | Protein ID | WP_000554758.1 |
| Coordinates | 3576677..3576970 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| - (3571864) | 3571864..3571944 | - | 81 | NuclAT_12 | - | - |
| - (3571864) | 3571864..3571944 | - | 81 | NuclAT_12 | - | - |
| - (3571864) | 3571864..3571944 | - | 81 | NuclAT_12 | - | - |
| - (3571864) | 3571864..3571944 | - | 81 | NuclAT_12 | - | - |
| JSU13_RS17120 (3572540) | 3572540..3572998 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| JSU13_RS17125 (3573259) | 3573259..3574716 | + | 1458 | WP_001292994.1 | cytosol nonspecific dipeptidase | - |
| JSU13_RS17130 (3574773) | 3574773..3575294 | - | 522 | Protein_3393 | peptide chain release factor H | - |
| JSU13_RS17135 (3575290) | 3575290..3575496 | - | 207 | Protein_3394 | RtcB family protein | - |
| JSU13_RS17140 (3575814) | 3575814..3576266 | - | 453 | WP_001059892.1 | GNAT family N-acetyltransferase | - |
| JSU13_RS17145 (3576276) | 3576276..3576674 | - | 399 | WP_001263489.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| JSU13_RS17150 (3576677) | 3576677..3576970 | - | 294 | WP_000554758.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| JSU13_RS17155 (3577022) | 3577022..3578077 | - | 1056 | WP_001226164.1 | DNA polymerase IV | - |
| JSU13_RS17160 (3578148) | 3578148..3578933 | - | 786 | WP_000207552.1 | putative lateral flagellar export/assembly protein LafU | - |
| JSU13_RS17165 (3578905) | 3578905..3580617 | + | 1713 | Protein_3400 | flagellar biosynthesis protein FlhA | - |
| JSU13_RS17170 (3580841) | 3580841..3581338 | - | 498 | WP_000006255.1 | REP-associated tyrosine transposase RayT | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15487.84 Da Isoelectric Point: 7.4215
>T191566 WP_001263489.1 NZ_CP069666:c3576674-3576276 [Escherichia coli O89m:H9]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T191566 NZ_CP091806:17533-17844 [Klebsiella quasipneumoniae]
GTGCATGTTATATCACGAGCCCCTTTTGATGAGGCGGCAAGACACTATCCGAATGATGCAGTGGCCATCGATGACACCTA
TCGTGTACTAAAGCGGGTTGTCGCAAAAAAACCGGATGAGCTAAAGCGATACTTTACCAGTCTGGATCGCATGAAATACC
GGGAAAAATGGTGGGTTATCGATATCGGTGGTAATAACCTTAGAATGATGTTTTTTGCTGATTTTGAGCGCGGAAAGATA
TTCGTCAAACACATCACCACCCATGCTGAATATGATCGCTTAACGAAATATTATCGGGAGCACAAAGAATGA
GTGCATGTTATATCACGAGCCCCTTTTGATGAGGCGGCAAGACACTATCCGAATGATGCAGTGGCCATCGATGACACCTA
TCGTGTACTAAAGCGGGTTGTCGCAAAAAAACCGGATGAGCTAAAGCGATACTTTACCAGTCTGGATCGCATGAAATACC
GGGAAAAATGGTGGGTTATCGATATCGGTGGTAATAACCTTAGAATGATGTTTTTTGCTGATTTTGAGCGCGGAAAGATA
TTCGTCAAACACATCACCACCCATGCTGAATATGATCGCTTAACGAAATATTATCGGGAGCACAAAGAATGA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.76 Da Isoelectric Point: 5.2170
>AT191566 WP_000554758.1 NZ_CP069666:c3576970-3576677 [Escherichia coli O89m:H9]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT191566 NZ_CP091806:17841-18257 [Klebsiella quasipneumoniae]
ATGATTTTTAGCGATGCCATTAAAGCGGCGAATGACCTGGCCAGTATTGTCCCTCTGCTGGGTGGCAGCTCGTCCCGCAA
AGATTACGAAGAGGCACTTAAACTCGTTGAATATCTTCTGGAACATGACCCGGACAGCCCGCTGGTTGATATGCTGACTG
CCCGCATTGATGCCTGGGAAGATAACGCGGTGGAATTTGAAGAATTTAATACGCGCATCGAAGCAGGCAAGAACGGTGTT
TCGTTGCTGCGGGTTCTCATGCAGCAACACGGGCTTTCACAGTCCGATTTTGAAAACGAGATAGGCAAAAAATCGCTCGT
CAGCCGGATCCTCAGTGGTGAACGTAGTCTGACCCTCGATCACATGCGGGCGCTGGCAAATCGATTTCAAATCCCGGTCT
CGATGTTTGTTGACTGA
ATGATTTTTAGCGATGCCATTAAAGCGGCGAATGACCTGGCCAGTATTGTCCCTCTGCTGGGTGGCAGCTCGTCCCGCAA
AGATTACGAAGAGGCACTTAAACTCGTTGAATATCTTCTGGAACATGACCCGGACAGCCCGCTGGTTGATATGCTGACTG
CCCGCATTGATGCCTGGGAAGATAACGCGGTGGAATTTGAAGAATTTAATACGCGCATCGAAGCAGGCAAGAACGGTGTT
TCGTTGCTGCGGGTTCTCATGCAGCAACACGGGCTTTCACAGTCCGATTTTGAAAACGAGATAGGCAAAAAATCGCTCGT
CAGCCGGATCCTCAGTGGTGAACGTAGTCTGACCCTCGATCACATGCGGGCGCTGGCAAATCGATTTCAAATCCCGGTCT
CGATGTTTGTTGACTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A090J8B1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | S1QAE3 |