Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 645992..646719 Replicon chromosome
Accession NZ_CP069666
Organism Escherichia coli O89m:H9 strain MIN11

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag JSU13_RS03145 Protein ID WP_000550189.1
Coordinates 645992..646306 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JSU13_RS03150 Protein ID WP_000560266.1
Coordinates 646303..646719 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU13_RS03125 (642150) 642150..643136 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
JSU13_RS03130 (643215) 643215..643907 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
JSU13_RS03135 (643984) 643984..644487 - 504 WP_001333820.1 M48 family metallopeptidase -
JSU13_RS03140 (644572) 644572..645708 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
JSU13_RS03145 (645992) 645992..646306 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
JSU13_RS03150 (646303) 646303..646719 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
JSU13_RS03155 (646764) 646764..648782 - 2019 WP_208207762.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
JSU13_RS03160 (649208) 649208..651559 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T191549 WP_000550189.1 NZ_CP069666:645992-646306 [Escherichia coli O89m:H9]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T191549 NZ_CP091779:c2746091-2745988 [Enterobacter mori]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT191549 WP_000560266.1 NZ_CP069666:646303-646719 [Escherichia coli O89m:H9]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT191549 NZ_CP091779:2745983-2746126 [Enterobacter mori]
CAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTAAGTCGCCTTGCCTTTTAAGAATAGATGACGACGCCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References