Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 602646..603445 Replicon chromosome
Accession NZ_CP069666
Organism Escherichia coli O89m:H9 strain MIN11

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag JSU13_RS02925 Protein ID WP_000347275.1
Coordinates 602646..603110 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JSU13_RS02930 Protein ID WP_001307405.1
Coordinates 603110..603445 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU13_RS02895 (597647) 597647..598081 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
JSU13_RS02900 (598099) 598099..598977 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
JSU13_RS02905 (598967) 598967..599746 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
JSU13_RS02910 (599757) 599757..600230 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
JSU13_RS02915 (600253) 600253..601533 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
JSU13_RS02920 (601782) 601782..602591 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JSU13_RS02925 (602646) 602646..603110 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JSU13_RS02930 (603110) 603110..603445 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JSU13_RS02935 (603594) 603594..605165 - 1572 WP_001273752.1 galactarate dehydratase -
JSU13_RS02940 (605540) 605540..606874 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
JSU13_RS02945 (606890) 606890..607660 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T191548 WP_000347275.1 NZ_CP069666:c603110-602646 [Escherichia coli O89m:H9]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T191548 NZ_CP091779:c1055233-1055015 [Enterobacter mori]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCTTGCGACGTTGTCAGTCAATAGACACGCTCGAACGTGT
CATCGAAAAAAATAAATACGAGCTGTCTGATAATGAACTGGCTGTATTTTATTCAGCCGCTGACCATCGGCTCGCTGAGC
TGACCATGAATAAGCTGTATGACAAAATCCCAACGTCCGTATGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT191548 WP_001307405.1 NZ_CP069666:c603445-603110 [Escherichia coli O89m:H9]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT191548 NZ_CP091779:c1055635-1055261 [Enterobacter mori]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTGAATGACCCAACGTCTGCGATCAATTTACAGTTGAACGAGCTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGACGAATACCTG
GACGACACCTTTATGTTGTTCAGTAGCTACGGCATTAACGCCCAGGATTTGCAAAAATGGCGTAAATCGGGAAATCGGCT
ATTCCGCTGCTTCGTCAACGTGAGCAGAGCAAACCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References