Detailed information of TA system TA191541

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 134729..135155
• Replicon: plasmid pMUB-MIN12-1
• Accession: NZ_CP069658
• Organism: Escherichia coli O89m:H10 strain MIN12

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: JSU14_RS24235
• Protein ID: WP_001372321.1
• Coordinates: 134729..134854 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	134931..135155 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSU14_RS24200 (129775)	129775..130002	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
JSU14_RS24205 (130096)	130096..130782	-	687	WP_000332484.1	PAS domain-containing protein	-
JSU14_RS24210 (130973)	130973..131356	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
JSU14_RS24215 (131633)	131633..132280	+	648	WP_000614936.1	transglycosylase SLT domain-containing protein	-
JSU14_RS24220 (132577)	132577..133398	-	822	WP_001234469.1	DUF932 domain-containing protein	-
JSU14_RS24225 (133520)	133520..133807	-	288	WP_000107535.1	hypothetical protein	-
JSU14_RS24230 (133832)	133832..134038	-	207	WP_000547939.1	hypothetical protein	-
JSU14_RS26000 (134108)	134108..134281	+	174	Protein_165	hypothetical protein	-
JSU14_RS26005 (134279)	134279..134509	-	231	WP_001426396.1	hypothetical protein	-
JSU14_RS24235 (134729)	134729..134854	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
JSU14_RS26010 (134796)	134796..134945	-	150	Protein_168	plasmid maintenance protein Mok	-
- (134931)	134931..135155	-	225	NuclAT_0	-	Antitoxin
- (134931)	134931..135155	-	225	NuclAT_0	-	Antitoxin
- (134931)	134931..135155	-	225	NuclAT_0	-	Antitoxin
- (134931)	134931..135155	-	225	NuclAT_0	-	Antitoxin
JSU14_RS24240 (134967)	134967..135155	+	189	WP_001299721.1	hypothetical protein	-
JSU14_RS24245 (135124)	135124..135886	-	763	Protein_170	plasmid SOS inhibition protein A	-
JSU14_RS24250 (135883)	135883..136317	-	435	WP_000845895.1	conjugation system SOS inhibitor PsiB	-
JSU14_RS24255 (136372)	136372..138330	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
JSU14_RS24260 (138389)	138389..138622	-	234	WP_000006004.1	DUF905 domain-containing protein	-
JSU14_RS24265 (138678)	138678..139205	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
JSU14_RS24270 (139678)	139678..139950	-	273	WP_072133095.1	hypothetical protein	-
JSU14_RS26015 (139848)	139848..140102	-	255	WP_050195272.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(3')-Ia / tet(A) / sitABCD	-	1..163427	163427

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T191541 WP_001372321.1 NZ_CP069658:c134854-134729 [Escherichia coli O89m:H10]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T191541 NZ_CP091773:c4284907-4284734 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT191541 NZ_CP069658:c135155-134931 [Escherichia coli O89m:H10]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin