Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3783704..3784245 Replicon chromosome
Accession NZ_CP069657
Organism Escherichia coli O89m:H10 strain MIN12

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag JSU14_RS18180 Protein ID WP_000615983.1
Coordinates 3783967..3784245 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag JSU14_RS18175 Protein ID WP_000729703.1
Coordinates 3783704..3783964 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSU14_RS18155 (3779379) 3779379..3780164 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
JSU14_RS18160 (3780136) 3780136..3781848 + 1713 Protein_3598 flagellar biosynthesis protein FlhA -
JSU14_RS18165 (3782072) 3782072..3782569 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
JSU14_RS18170 (3782745) 3782745..3783494 - 750 WP_000056849.1 C40 family peptidase -
JSU14_RS18175 (3783704) 3783704..3783964 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JSU14_RS18180 (3783967) 3783967..3784245 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JSU14_RS18185 (3784401) 3784401..3785141 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
JSU14_RS18190 (3785112) 3785112..3785879 - 768 WP_000333380.1 class II glutamine amidotransferase -
JSU14_RS18195 (3786085) 3786085..3786663 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3777507..3792787 15280


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T191538 WP_000615983.1 NZ_CP069657:3783967-3784245 [Escherichia coli O89m:H10]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T191538 NZ_CP091773:2690733-2690840 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT191538 WP_000729703.1 NZ_CP069657:3783704-3783964 [Escherichia coli O89m:H10]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT191538 NZ_CP091773:c2690686-2690620 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References