Detailed information of TA system TA191520

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 719547..720240
• Replicon: chromosome
• Accession: NZ_CP069657
• Organism: Escherichia coli O89m:H10 strain MIN12

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: U9ZN09
• Locus tag: JSU14_RS03480
• Protein ID: WP_000415585.1
• Coordinates: 719547..719843 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: JSU14_RS03485
• Protein ID: WP_000650107.1
• Coordinates: 719845..720240 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSU14_RS25810 (714681)	714681..715160	+	480	WP_000065332.1	Hcp family type VI secretion system effector	-
JSU14_RS25815 (715163)	715163..715873	+	711	WP_000834030.1	hypothetical protein	-
JSU14_RS03455 (715880)	715880..716212	+	333	WP_000914690.1	DUF2645 family protein	-
JSU14_RS03460 (716258)	716258..717607	-	1350	WP_000673358.1	quorum sensing histidine kinase QseC	-
JSU14_RS03465 (717604)	717604..718263	-	660	WP_001221502.1	quorum sensing response regulator transcription factor QseB	-
JSU14_RS03470 (718415)	718415..718807	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
JSU14_RS03475 (718860)	718860..719342	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
JSU14_RS03480 (719547)	719547..719843	+	297	WP_000415585.1	type II toxin-antitoxin system toxin MqsR	Toxin
JSU14_RS03485 (719845)	719845..720240	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
JSU14_RS03490 (720373)	720373..721980	+	1608	WP_001375094.1	ABC transporter substrate-binding protein	-
JSU14_RS03495 (722118)	722118..724376	+	2259	WP_001281841.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (2-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11261.99 Da
• Isoelectric Point: 8.9070

>T191520 WP_000415585.1 NZ_CP069657:719547-719843 [Escherichia coli O89m:H10]
MEKRTPHTRLSQVKKLVNTGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T191520 NZ_CP091765:2317442-2318179 [Bacillus anthracis]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTAGTTCTTGTTTTCTTTTACTGGAGAAATGA
AGAATTGTATGAGGAGAAGAAGCAACGAATTAGAAAGACTTGGTATGGGTTGTTTATAGTATCAGTCACCGTGTATTTCA
TGATAAAAGGAATCGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATATTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATCATTCAAAATGTCAATCCAGCAGTGTTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGCATTTTTAGATTCGTATGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTACGATTATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAGAGCAGTACAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGATGGGAAGAAAAATAACCTGCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT191520 WP_000650107.1 NZ_CP069657:719845-720240 [Escherichia coli O89m:H10]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT191520 NZ_CP091765:2318292-2318417 [Bacillus anthracis]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCGTTAATAAAAGGAGATAC
ATTACAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATGA

Structures

Toxin

• AlphaFold DB: U9ZN09

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65