Detailed information of TA system TA191480

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2022636..2023435
• Replicon: chromosome
• Accession: NZ_CP069642
• Organism: Escherichia coli strain SSGNw2

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: JSQ95_RS09530
• Protein ID: WP_000347252.1
• Coordinates: 2022971..2023435 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: JSQ95_RS09525
• Protein ID: WP_001296435.1
• Coordinates: 2022636..2022971 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSQ95_RS09510 (2018421)	2018421..2019191	-	771	WP_248414242.1	2-dehydro-3-deoxyglucarate aldolase	-
JSQ95_RS09515 (2019207)	2019207..2020541	-	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
JSQ95_RS09520 (2020916)	2020916..2022487	+	1572	WP_001273940.1	galactarate dehydratase	-
JSQ95_RS09525 (2022636)	2022636..2022971	+	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
JSQ95_RS09530 (2022971)	2022971..2023435	+	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
JSQ95_RS09535 (2023490)	2023490..2024299	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
JSQ95_RS09540 (2024548)	2024548..2025828	+	1281	WP_000681926.1	tagatose-bisphosphate aldolase subunit KbaZ	-
JSQ95_RS09545 (2025851)	2025851..2026324	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
JSQ95_RS09550 (2026335)	2026335..2027114	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
JSQ95_RS09555 (2027104)	2027104..2027982	+	879	WP_001298314.1	PTS N-acetylgalactosamine transporter subunit IID	-
JSQ95_RS09560 (2028000)	2028000..2028434	+	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T191480 WP_000347252.1 NZ_CP069642:2022971-2023435 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T191480 NZ_CP069642:2022971-2023435 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGCGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAGCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT191480 WP_001296435.1 NZ_CP069642:2022636-2022971 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT191480 NZ_CP069642:2022636-2022971 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCTGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5