Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1445097..1445824 Replicon chromosome
Accession NZ_CP069630
Organism Escherichia coli strain ESM2.2

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag JSQ94_RS06820 Protein ID WP_000547555.1
Coordinates 1445513..1445824 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag JSQ94_RS06815 Protein ID WP_000126293.1
Coordinates 1445097..1445516 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ94_RS06800 (1441260) 1441260..1443512 - 2253 WP_001107659.1 carbamoyltransferase HypF -
JSQ94_RS06805 (1443665) 1443665..1444192 - 528 WP_001078777.1 electron transport protein HydN -
JSQ94_RS06810 (1444577) 1444577..1444876 + 300 WP_001704025.1 DUF4259 domain-containing protein -
JSQ94_RS06815 (1445097) 1445097..1445516 - 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
JSQ94_RS06820 (1445513) 1445513..1445824 - 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
JSQ94_RS06825 (1445987) 1445987..1446742 - 756 WP_001305268.1 hypothetical protein -
JSQ94_RS06830 (1446785) 1446785..1447255 - 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
JSQ94_RS06835 (1447248) 1447248..1447658 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
JSQ94_RS06840 (1447655) 1447655..1448422 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
JSQ94_RS06845 (1448422) 1448422..1448964 - 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
JSQ94_RS06850 (1448974) 1448974..1450683 - 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T191369 WP_000547555.1 NZ_CP069630:c1445824-1445513 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T191369 NZ_CP091691:c2965713-2965339 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT191369 WP_000126293.1 NZ_CP069630:c1445516-1445097 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT191369 NZ_CP091691:c2966171-2965803 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References