Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 68199..68761 Replicon chromosome
Accession NZ_CP069630
Organism Escherichia coli strain ESM2.2

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag JSQ94_RS00330 Protein ID WP_000605675.1
Coordinates 68483..68761 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag JSQ94_RS00325 Protein ID WP_000781370.1
Coordinates 68199..68483 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ94_RS00310 (63380) 63380..66427 + 3048 WP_248414188.1 formate dehydrogenase-N subunit alpha -
JSQ94_RS00315 (66440) 66440..67324 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
JSQ94_RS00320 (67317) 67317..67970 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
JSQ94_RS00325 (68199) 68199..68483 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
JSQ94_RS00330 (68483) 68483..68761 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JSQ94_RS00335 (68947) 68947..69957 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
JSQ94_RS00340 (70091) 70091..71788 - 1698 WP_148983813.1 malate dehydrogenase -
JSQ94_RS00345 (71945) 71945..72082 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
JSQ94_RS00350 (72184) 72184..72399 - 216 WP_000495766.1 biofilm-dependent modulation protein -
JSQ94_RS00355 (72744) 72744..73175 + 432 WP_000152307.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T191357 WP_000605675.1 NZ_CP069630:c68761-68483 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T191357 NZ_CP091691:652821-653072 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT191357 WP_000781370.1 NZ_CP069630:c68483-68199 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT191357 NZ_CP091691:653069-653323 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCTCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCTACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References