191345

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2022636..2023435 Replicon chromosome
Accession NZ_CP069627
Organism Escherichia coli strain ESM1.4

Toxin (Protein)


Gene name yhaV Uniprot ID Q8FDB4
Locus tag JSQ89_RS09535 Protein ID WP_000347252.1
Coordinates 2022971..2023435 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag JSQ89_RS09530 Protein ID WP_001296435.1
Coordinates 2022636..2022971 (+) Length 112 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ89_RS09515 (2018421) 2018421..2019191 - 771 WP_001058223.1 2-dehydro-3-deoxyglucarate aldolase -
JSQ89_RS09520 (2019207) 2019207..2020541 - 1335 WP_000979025.1 galactarate/glucarate/glycerate transporter GarP -
JSQ89_RS09525 (2020916) 2020916..2022487 + 1572 WP_001273940.1 galactarate dehydratase -
JSQ89_RS09530 (2022636) 2022636..2022971 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JSQ89_RS09535 (2022971) 2022971..2023435 + 465 WP_000347252.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JSQ89_RS09540 (2023490) 2023490..2024299 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JSQ89_RS09545 (2024548) 2024548..2025828 + 1281 WP_000681926.1 tagatose-bisphosphate aldolase subunit KbaZ -
JSQ89_RS09550 (2025851) 2025851..2026324 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JSQ89_RS09555 (2026335) 2026335..2027114 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
JSQ89_RS09560 (2027104) 2027104..2027982 + 879 WP_001298314.1 PTS N-acetylgalactosamine transporter subunit IID -
JSQ89_RS09565 (2028000) 2028000..2028434 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-150)

Antitoxin

(14-110)


Sequences


Toxin        


Download         Length: 155 a.a.        Molecular weight: 17750.11 Da        Isoelectric Point: 9.4947

>T191345 WP_000347252.1 NZ_CP069627:2022971-2023435 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Download         Length: 465 bp

>T191345 NZ_CP091682:c2838609-2838355 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT191345 WP_001296435.1 NZ_CP069627:2022636-2022971 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT191345 NZ_CP091682:c2838857-2838606 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB Q8FDB4


Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References