Detailed information of TA system TA191075

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1416626..1417218
• Replicon: chromosome
• Accession: NZ_CP069594
• Organism: Pseudomonas sp. Y39-6

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: JN756_RS06325
• Protein ID: WP_201021939.1
• Coordinates: 1416626..1416904 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: A0A1D3JSM0
• Locus tag: JN756_RS06330
• Protein ID: WP_017844583.1
• Coordinates: 1416913..1417218 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN756_RS06305 (JN756_06240)	1411711..1412085	+	375	WP_046384565.1	GtrA family protein	-
JN756_RS06310 (JN756_06245)	1412082..1413056	+	975	WP_046384564.1	glycosyltransferase family 2 protein	-
JN756_RS06315 (JN756_06250)	1413053..1414624	+	1572	WP_201021937.1	glycosyltransferase family 39 protein	-
JN756_RS06320 (JN756_06255)	1414703..1416322	-	1620	WP_201021938.1	FMN-binding glutamate synthase family protein	-
JN756_RS06325 (JN756_06260)	1416626..1416904	+	279	WP_201021939.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JN756_RS06330 (JN756_06265)	1416913..1417218	+	306	WP_017844583.1	HigA family addiction module antitoxin	Antitoxin
JN756_RS06335 (JN756_06270)	1417299..1418690	-	1392	WP_079442937.1	ATP-binding protein	-
JN756_RS06340 (JN756_06275)	1418687..1420501	-	1815	WP_046384560.1	ATP-binding protein	-
JN756_RS06345 (JN756_06280)	1420512..1421498	-	987	WP_108525880.1	cytochrome c peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10569.02 Da
• Isoelectric Point: 7.2444

>T191075 WP_201021939.1 NZ_CP069594:1416626-1416904 [Pseudomonas sp. Y39-6]
MIRSFKCAETQKLFETGRTRRWSAVVSVVERQLSMLDAAVELADLRSPPGNRLEPLYGDREGQHSIRINGQFRVCFIWGP
HGPENVEIVDYH

Nucleotide

• Length: 279 bp

>T191075 NZ_CP091634:3342482-3342700 [Salmonella enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11115.93 Da
• Isoelectric Point: 7.2767

>AT191075 WP_017844583.1 NZ_CP069594:1416913-1417218 [Pseudomonas sp. Y39-6]
MFKNGMRPVHPGEILKEEYLEPLGLTAAALARALHVSAPTVNDIVLMRRSISADVALRLAVALETSAQFWLNLQTAYDLR
TAEIAKGAQIYKEVERVAHCA

Nucleotide

• Length: 306 bp

>AT191075 NZ_CP091634:3342080-3342454 [Salmonella enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A1D3JSM0