Detailed information of TA system TA191070

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 85500..85926
• Replicon: plasmid unnamed1
• Accession: NZ_CP069590
• Organism: Escherichia coli strain FDAARGOS_1266

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: I6J96_RS25145
• Protein ID: WP_001312861.1
• Coordinates: 85768..85926 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	85500..85724 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J96_RS25115	81450..81977	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
I6J96_RS25120	82033..82266	+	234	WP_000006004.1	DUF905 domain-containing protein	-
I6J96_RS25125	82325..84283	+	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
I6J96_RS25130	84338..84772	+	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
I6J96_RS25135	84769..85488	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
I6J96_RS25140	85500..85688	-	189	WP_001299721.1	hypothetical protein	-
-	85500..85724	+	225	NuclAT_0	-	Antitoxin
-	85500..85724	+	225	NuclAT_0	-	Antitoxin
-	85500..85724	+	225	NuclAT_0	-	Antitoxin
-	85500..85724	+	225	NuclAT_0	-	Antitoxin
I6J96_RS25145	85768..85926	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
I6J96_RS25150	86845..87132	+	288	WP_000107537.1	hypothetical protein	-
I6J96_RS25155	87253..88074	+	822	WP_204097655.1	DUF945 domain-containing protein	-
I6J96_RS25160	88371..89018	-	648	WP_000614282.1	transglycosylase SLT domain-containing protein	-
I6J96_RS25165	89304..89687	+	384	WP_001151564.1	relaxosome protein TraM	-
I6J96_RS25170	89881..90567	+	687	WP_000332487.1	PAS domain-containing protein	-
I6J96_RS25175	90661..90888	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib-cr / blaOXA-1 / blaTEM-1B / tet(A) / blaCTX-M-15	-	1..127965	127965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T191070 WP_001312861.1 NZ_CP069590:85768-85926 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T191070 NZ_CP091634:213723-214208 [Salmonella enterica]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGGGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT191070 NZ_CP069590:85500-85724 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin