Detailed information of TA system TA191065

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 4714628..4715307
• Replicon: chromosome
• Accession: NZ_CP069589
• Organism: Escherichia coli strain FDAARGOS_1266

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1PK60
• Locus tag: I6J96_RS22910
• Protein ID: WP_000057523.1
• Coordinates: 4714628..4714930 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: S1QAY3
• Locus tag: I6J96_RS22915
• Protein ID: WP_000806442.1
• Coordinates: 4714966..4715307 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J96_RS22885	4710004..4711656	+	1653	WP_000771748.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
I6J96_RS22890	4711694..4712197	-	504	WP_000667000.1	hypothetical protein	-
I6J96_RS22895	4712194..4712994	-	801	WP_000439798.1	hypothetical protein	-
I6J96_RS22900	4713018..4713497	-	480	WP_000186633.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
I6J96_RS22905	4713701..4714495	-	795	WP_000365147.1	TraB/GumN family protein	-
I6J96_RS22910	4714628..4714930	+	303	WP_000057523.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6J96_RS22915	4714966..4715307	+	342	WP_000806442.1	HigA family addiction module antidote protein	Antitoxin
I6J96_RS22920	4715365..4717869	-	2505	WP_000083947.1	copper-exporting P-type ATPase CopA	-
I6J96_RS22925	4718131..4719063	+	933	WP_000883041.1	glutaminase A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11809.44 Da
• Isoelectric Point: 10.3980

>T191065 WP_000057523.1 NZ_CP069589:4714628-4714930 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Nucleotide

• Length: 303 bp

>T191065 NZ_CP091630:3538555-3538800 [Salmonella enterica]
ATGAATGCCTCCGACGTCAGCGTCCGCCACATCAACAGCGAAACCTGCATGACCGCCTGTTACTCACAAATCCCCAGCGG
CGACTGCCAGGAAGAAGCGGGATTTGAGACCGGGCGCAGCGTGACGGTGAAGATTTCGGACGGGTGTATTGTGCTAATGG
CGGACGGTAACGAGGTGCAGAAGCTGTGCGAACAGCTTTATCAGGCTGAACAGGTAGTTAAGGGGATGTGGGACGTGATT
GTTTAG

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13171.10 Da
• Isoelectric Point: 5.7790

>AT191065 WP_000806442.1 NZ_CP069589:4714966-4715307 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT191065 NZ_CP091630:c3538764-3538408 [Salmonella enterica]
TTCAGCCTGATAAAGCTGTTCGCACAGCTTCTGCACCTCGTTACCGTCCGCCATTAGCACAATACACCCGTCCGAAATCT
TCACCGTCACGCTGCGCCCGGTCTCAAATCCCGCTTCTTCCTGGCAGTCGCCGCTGGGGATTTGTGAGTAACAGGCGGTC
ATGCAGGTTTCGCTGTTGATGTGGCGGACGCTGACGTCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTCTC
CGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTGAGCGAGTATGGGCGCGACATCCTGGCATCGCGCCTCTCTCTCCCCG
GCCAGTGATGAGCAACCGGCTGCCGGGGCCGTATTTT

Structures

Toxin

• AlphaFold DB: S1PK60

Antitoxin

• AlphaFold DB: S1QAY3