Detailed information of TA system TA191049

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1383400..1383902
• Replicon: chromosome
• Accession: NZ_CP069589
• Organism: Escherichia coli strain FDAARGOS_1266

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: I6J96_RS07235
• Protein ID: WP_000767819.1
• Coordinates: 1383400..1383654 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: I6J96_RS07240
• Protein ID: WP_001259253.1
• Coordinates: 1383651..1383902 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J96_RS07210	1379218..1379676	-	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-
I6J96_RS07215	1379893..1381251	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
I6J96_RS07220	1381241..1381303	-	63	WP_010723108.1	membrane protein YoeI	-
I6J96_RS07225	1381518..1382447	-	930	WP_000803366.1	LysR family transcriptional regulator	-
I6J96_RS07230	1382493..1383317	-	825	WP_000754737.1	SDR family oxidoreductase	-
I6J96_RS07235	1383400..1383654	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
I6J96_RS07240	1383651..1383902	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
I6J96_RS07245	1384185..1384235	+	51	WP_001364200.1	his operon leader peptide	-
I6J96_RS07250	1384381..1385280	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
I6J96_RS07255	1385286..1386590	+	1305	WP_001296211.1	histidinol dehydrogenase	-
I6J96_RS07260	1386587..1387657	+	1071	WP_000108967.1	histidinol-phosphate transaminase	-
I6J96_RS07265	1387657..1388724	+	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1379218..1379676	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T191049 WP_000767819.1 NZ_CP069589:c1383654-1383400 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T191049 NZ_CP091626:317650-318003 [Salmonella enterica]
ATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGGCATGCCTGATCCTGG
GCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGTGGCTGCTGGCGGCAA
TGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTTATTACGCTGCTGTTT
TTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGCGGCAGGGCGGCTTAC
GCTGGAGCAAATTGTCGTTCGCTTACGCGCCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT191049 WP_001259253.1 NZ_CP069589:c1383902-1383651 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT191049 NZ_CP091626:317416-317637 [Salmonella enterica]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3