Detailed information of TA system TA191029

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 97795..98049
• Replicon: plasmid unnamed1
• Accession: NZ_CP069584
• Organism: Escherichia coli strain FDAARGOS_1265

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: I6J95_RS26095
• Protein ID: WP_015387399.1
• Coordinates: 97795..98001 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	97988..98049 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J95_RS26065	93042..93953	-	912	WP_000440183.1	carbamate kinase	-
I6J95_RS26070	93964..95184	-	1221	WP_000410951.1	arginine deiminase	-
I6J95_RS26075	96077..96559	+	483	Protein_112	VENN motif pre-toxin domain-containing protein	-
I6J95_RS26080	96505..96951	-	447	Protein_113	incFII family plasmid replication initiator RepA	-
I6J95_RS26085	96944..97018	-	75	WP_032336874.1	RepA leader peptide Tap	-
I6J95_RS26090	97254..97511	-	258	WP_000083833.1	replication regulatory protein RepA	-
I6J95_RS26095	97795..98001	-	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	97988..98049	+	62	NuclAT_2	-	Antitoxin
-	97988..98049	+	62	NuclAT_2	-	Antitoxin
-	97988..98049	+	62	NuclAT_2	-	Antitoxin
-	97988..98049	+	62	NuclAT_2	-	Antitoxin
I6J95_RS26100	98188..98370	-	183	WP_000968309.1	hypothetical protein	-
I6J95_RS26105	98471..99076	+	606	Protein_118	IS1 family transposase	-
I6J95_RS26110	99114..100685	-	1572	WP_023149734.1	IS66 family transposase	-
I6J95_RS26115	100705..101052	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
I6J95_RS26120	101052..101729	-	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
I6J95_RS26125	101784..101873	+	90	Protein_122	IS1 family transposase	-
I6J95_RS26130	102174..102386	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A)	iucD / iutA / senB	1..206267	206267

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T191029 WP_015387399.1 NZ_CP069584:c98001-97795 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T191029 NZ_CP091622:317650-318003 [Salmonella enterica]
ATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGGCATGCCTGATCCTGG
GCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGTGGCTGCTGGCGGCAA
TGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTTATTACGCTGCTGTTT
TTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGCGGCAGGGCGGCTTAC
GCTGGAGCAAATTGTCGTTCGCTTACGCGCCTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT191029 NZ_CP069584:97988-98049 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin