Detailed information of TA system TA191023

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 7292..7525
• Replicon: plasmid unnamed1
• Accession: NZ_CP069584
• Organism: Escherichia coli strain FDAARGOS_1265

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: I6J95_RS25580
• Protein ID: WP_001312861.1
• Coordinates: 7367..7525 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	7292..7323 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J95_RS25540	2586..3065	-	480	Protein_5	recombinase family protein	-
I6J95_RS25545	3118..3822	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
I6J95_RS25550	3978..4211	+	234	WP_000005990.1	DUF905 family protein	-
I6J95_RS25555	4239..4436	+	198	Protein_8	hypothetical protein	-
I6J95_RS25560	4491..4925	+	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
I6J95_RS25565	4922..5641	+	720	WP_001276238.1	plasmid SOS inhibition protein A	-
I6J95_RS25570	5653..5841	-	189	WP_001299721.1	hypothetical protein	-
-	5653..5850	+	198	NuclAT_0	-	-
-	5653..5850	+	198	NuclAT_0	-	-
-	5653..5850	+	198	NuclAT_0	-	-
-	5653..5850	+	198	NuclAT_0	-	-
I6J95_RS25575	5898..7267	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
-	7292..7323	+	32	NuclAT_1	-	Antitoxin
-	7292..7323	+	32	NuclAT_1	-	Antitoxin
-	7292..7323	+	32	NuclAT_1	-	Antitoxin
-	7292..7323	+	32	NuclAT_1	-	Antitoxin
I6J95_RS25580	7367..7525	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
I6J95_RS25585	8446..8733	+	288	WP_000107535.1	hypothetical protein	-
I6J95_RS25590	8851..9672	+	822	WP_001234426.1	DUF945 domain-containing protein	-
I6J95_RS25595	9969..10571	-	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
I6J95_RS25600	10892..11275	+	384	WP_001151524.1	relaxosome protein TraM	-
I6J95_RS25605	11462..12151	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A)	iucD / iutA / senB	1..206267	206267

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T191023 WP_001312861.1 NZ_CP069584:7367-7525 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T191023 NZ_CP091619:3497187-3497432 [Salmonella enterica]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCGAAACCTGCATGACCGCCTGTTACTCACAAATCCCCAGCGG
CGACTGCCAGGAAGAAGCGGGATTTGAGACCGGGCGCAGCGTGACGGTGAAGATTTCGGACGGGTGTATTGTGCTAATGG
CGGACGGTAACGAGGTGCAGAAGCTGTGCGAACAGCTTTATCAGGCTGAACAGGTAGTTAAGGGGATGTGGGACGTGATT
GTTTAG

Antitoxin

Nucleotide

• Length: 32 bp

>AT191023 NZ_CP069584:7292-7323 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin