Detailed information of TA system TA191022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4903087..4903689
• Replicon: chromosome
• Accession: NZ_CP069583
• Organism: Escherichia coli strain FDAARGOS_1265

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: I6J95_RS24030
• Protein ID: WP_000897302.1
• Coordinates: 4903378..4903689 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6J95_RS24025
• Protein ID: WP_000356397.1
• Coordinates: 4903087..4903377 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J95_RS23995	4898394..4899323	+	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-
I6J95_RS24000	4899505..4899747	-	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
I6J95_RS24005	4900037..4900885	+	849	WP_001038650.1	hypothetical protein	-
I6J95_RS24010	4901201..4901650	+	450	WP_032140890.1	hypothetical protein	-
I6J95_RS24015	4901835..4902053	-	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
I6J95_RS24020	4902450..4902728	-	279	WP_001296612.1	hypothetical protein	-
I6J95_RS24025	4903087..4903377	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
I6J95_RS24030	4903378..4903689	-	312	WP_000897302.1	hypothetical protein	Toxin
I6J95_RS24035	4903918..4904826	+	909	WP_001385591.1	alpha/beta hydrolase	-
I6J95_RS24040	4904890..4905831	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6J95_RS24045	4905876..4906313	-	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
I6J95_RS24050	4906310..4907182	-	873	WP_000920762.1	virulence factor BrkB family protein	-
I6J95_RS24055	4907176..4907775	-	600	WP_001296610.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T191022 WP_000897302.1 NZ_CP069583:c4903689-4903378 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T191022 NZ_CP091619:3306402-3306620 [Salmonella enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT191022 WP_000356397.1 NZ_CP069583:c4903377-4903087 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT191022 NZ_CP091619:3306000-3306374 [Salmonella enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: S1P416