Detailed information of TA system TA190988

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 68712..69138
• Replicon: plasmid unnamed2
• Accession: NZ_CP069572
• Organism: Escherichia coli strain FDAARGOS_1250

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: I6J80_RS24315
• Protein ID: WP_001312861.1
• Coordinates: 68712..68870 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	68914..69138 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J80_RS24285	64084..64773	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
I6J80_RS24290	64960..65343	-	384	WP_001151524.1	relaxosome protein TraM	-
I6J80_RS24295	65664..66266	+	603	WP_072156291.1	transglycosylase SLT domain-containing protein	-
I6J80_RS24300	66563..67384	-	822	WP_001234469.1	DUF945 domain-containing protein	-
I6J80_RS24305	67503..67790	-	288	WP_000107535.1	hypothetical protein	-
I6J80_RS24310	67815..68021	-	207	WP_000547971.1	hypothetical protein	-
I6J80_RS24315	68712..68870	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	68914..69138	-	225	NuclAT_0	-	Antitoxin
-	68914..69138	-	225	NuclAT_0	-	Antitoxin
-	68914..69138	-	225	NuclAT_0	-	Antitoxin
-	68914..69138	-	225	NuclAT_0	-	Antitoxin
I6J80_RS24320	68950..69138	+	189	WP_001299721.1	hypothetical protein	-
I6J80_RS24325	69150..69869	-	720	WP_000116352.1	plasmid SOS inhibition protein A	-
I6J80_RS24330	69866..70300	-	435	WP_064770609.1	conjugation system SOS inhibitor PsiB	-
I6J80_RS24335	70355..70552	-	198	Protein_87	hypothetical protein	-
I6J80_RS24340	70580..70813	-	234	WP_000005987.1	DUF905 family protein	-
I6J80_RS24345	70881..71420	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
I6J80_RS24350	71446..71652	-	207	WP_000275856.1	hypothetical protein	-
I6J80_RS24355	72791..73762	-	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	floR / ant(3'')-Ia / sul3 / dfrA14 / aph(3')-Ia / aac(3)-IIa / sitABCD / blaCTX-M-55 / tet(A)	iutA / iucD / iucC / iucB / iucA	1..135042	135042

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T190988 WP_001312861.1 NZ_CP069572:c68870-68712 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T190988 NZ_CP091607:c1014615-1014088 [Salmonella enterica]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGAAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGGGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTACTG
TCGTTTAAAACGCTTTATGCGGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT190988 NZ_CP069572:c69138-68914 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin