Detailed information of TA system TA190983

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 30395..30649
• Replicon: plasmid unnamed2
• Accession: NZ_CP069572
• Organism: Escherichia coli strain FDAARGOS_1250

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: I6J80_RS24090
• Protein ID: WP_001351576.1
• Coordinates: 30395..30601 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	30588..30649 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J80_RS24055	25947..26348	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
I6J80_RS24060	26281..26538	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
I6J80_RS24065	26631..27284	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
I6J80_RS24070	28223..29080	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
I6J80_RS24075	29073..29555	-	483	WP_001273588.1	hypothetical protein	-
I6J80_RS24080	29548..29622	-	75	WP_001442103.1	RepA leader peptide Tap	-
I6J80_RS24085	29854..30111	-	258	WP_000083845.1	replication regulatory protein RepA	-
I6J80_RS24090	30395..30601	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	30588..30649	+	62	NuclAT_1	-	Antitoxin
-	30588..30649	+	62	NuclAT_1	-	Antitoxin
-	30588..30649	+	62	NuclAT_1	-	Antitoxin
-	30588..30649	+	62	NuclAT_1	-	Antitoxin
I6J80_RS24095	30905..30979	-	75	Protein_39	endonuclease	-
I6J80_RS24100	31225..31437	-	213	WP_001299730.1	ANR family transcriptional regulator	-
I6J80_RS24105	31573..32133	-	561	WP_000139315.1	fertility inhibition protein FinO	-
I6J80_RS24110	32236..33096	-	861	WP_000704513.1	alpha/beta hydrolase	-
I6J80_RS24115	33155..33901	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	floR / ant(3'')-Ia / sul3 / dfrA14 / aph(3')-Ia / aac(3)-IIa / sitABCD / blaCTX-M-55 / tet(A)	iutA / iucD / iucC / iucB / iucA	1..135042	135042

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T190983 WP_001351576.1 NZ_CP069572:c30601-30395 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T190983 NZ_CP091604:c4490969-4490658 [Salmonella enterica]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT190983 NZ_CP069572:30588-30649 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin