Detailed information of TA system TA190966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 1829807..1830500
• Replicon: chromosome
• Accession: NZ_CP069571
• Organism: Escherichia coli strain FDAARGOS_1250

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: I6J80_RS09315
• Protein ID: WP_000415584.1
• Coordinates: 1829807..1830103 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: I6J80_RS09320
• Protein ID: WP_000650107.1
• Coordinates: 1830105..1830500 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J80_RS09280	1824895..1825209	-	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-
I6J80_RS09285	1825240..1825821	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
I6J80_RS09290	1826140..1826472	+	333	WP_000917684.1	DUF2645 family protein	-
I6J80_RS09295	1826518..1827867	-	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
I6J80_RS09300	1827864..1828523	-	660	WP_139560537.1	two-component system response regulator QseB	-
I6J80_RS09305	1828675..1829067	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
I6J80_RS09310	1829120..1829602	+	483	WP_000183505.1	transcriptional regulator	-
I6J80_RS09315	1829807..1830103	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
I6J80_RS09320	1830105..1830500	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
I6J80_RS09325	1830633..1832240	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
I6J80_RS09330	1832378..1834636	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T190966 WP_000415584.1 NZ_CP069571:1829807-1830103 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T190966 NZ_CP091601:1148105-1148638 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT190966 WP_000650107.1 NZ_CP069571:1830105-1830500 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT190966 NZ_CP091601:1147828-1148094 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65