Detailed information of TA system TA190961

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 20907..21601
• Replicon: chromosome
• Accession: NZ_CP069571
• Organism: Escherichia coli strain FDAARGOS_1250

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: I6J80_RS00680
• Protein ID: WP_001263493.1
• Coordinates: 20907..21305 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: I6J80_RS00685
• Protein ID: WP_000554757.1
• Coordinates: 21308..21601 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J80_RS00650	15907..17151	-	1245	WP_000189541.1	esterase FrsA	-
-	16567..16647	-	81	NuclAT_13	-	-
-	16567..16647	-	81	NuclAT_13	-	-
-	16567..16647	-	81	NuclAT_13	-	-
-	16567..16647	-	81	NuclAT_13	-	-
I6J80_RS00655	17243..17701	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
I6J80_RS00660	17962..19419	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
I6J80_RS00665	19476..19997	-	522	Protein_27	peptide chain release factor H	-
I6J80_RS00670	19996..20199	-	204	Protein_28	RNA ligase RtcB family protein	-
I6J80_RS00675	20445..20897	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
I6J80_RS00680	20907..21305	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
I6J80_RS00685	21308..21601	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
I6J80_RS00690	21653..22708	-	1056	WP_001226164.1	DNA polymerase IV	-
I6J80_RS00695	22779..23564	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
I6J80_RS00700	23536..25248	+	1713	Protein_34	flagellar biosynthesis protein FlhA	-
I6J80_RS00705	25464..25961	-	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T190961 WP_001263493.1 NZ_CP069571:c21305-20907 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T190961 NZ_CP091599:189672-190154 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT190961 WP_000554757.1 NZ_CP069571:c21601-21308 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT190961 NZ_CP091599:189418-189684 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6