Detailed information of TA system TA190935

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 132929..133355
• Replicon: plasmid unnamed2
• Accession: NZ_CP069561
• Organism: Escherichia coli strain FDAARGOS_1259

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: I6J89_RS25375
• Protein ID: WP_001312861.1
• Coordinates: 132929..133087 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	133131..133355 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J89_RS25345	128326..129015	-	690	WP_000283387.1	conjugal transfer transcriptional regulator TraJ	-
I6J89_RS25350	129202..129585	-	384	WP_001151538.1	relaxosome protein TraM	-
I6J89_RS25355	129906..130508	+	603	Protein_155	transglycosylase SLT domain-containing protein	-
I6J89_RS25360	130805..131626	-	822	WP_001234445.1	DUF945 domain-containing protein	-
I6J89_RS25365	131736..132032	-	297	WP_001272251.1	hypothetical protein	-
I6J89_RS25370	132424..132628	+	205	Protein_158	pilus protein	-
I6J89_RS25375	132929..133087	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	133131..133355	-	225	NuclAT_0	-	Antitoxin
-	133131..133355	-	225	NuclAT_0	-	Antitoxin
-	133131..133355	-	225	NuclAT_0	-	Antitoxin
-	133131..133355	-	225	NuclAT_0	-	Antitoxin
I6J89_RS25380	133167..133346	+	180	WP_001309233.1	hypothetical protein	-
I6J89_RS25385	133367..134086	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
I6J89_RS25390	134083..134517	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
I6J89_RS25395	134586..136550	-	1965	WP_059342185.1	ParB/RepB/Spo0J family partition protein	-
I6J89_RS25400	136611..136844	-	234	WP_000006003.1	DUF905 family protein	-
I6J89_RS25405	136902..137429	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
I6J89_RS25410	137731..138192	+	462	WP_106426744.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A)	senB	1..144098	144098

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T190935 WP_001312861.1 NZ_CP069561:c133087-132929 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T190935 NZ_CP091582:c1764156-1763773 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT190935 NZ_CP069561:c133355-133131 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin