Detailed information of TA system TA190931

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 90551..90804
• Replicon: plasmid unnamed2
• Accession: NZ_CP069561
• Organism: Escherichia coli strain FDAARGOS_1259

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: I6J89_RS25125
• Protein ID: WP_001336447.1
• Coordinates: 90551..90700 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	90748..90804 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J89_RS25085	85629..86326	-	698	Protein_101	IS1 family transposase	-
I6J89_RS25090	86575..86976	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
I6J89_RS25095	86909..87166	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
I6J89_RS25100	87259..87912	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
I6J89_RS25105	88010..88150	-	141	WP_001333237.1	hypothetical protein	-
I6J89_RS25110	88851..89708	-	858	WP_001537577.1	incFII family plasmid replication initiator RepA	-
I6J89_RS25115	89701..89775	-	75	WP_001365705.1	RepA leader peptide Tap	-
I6J89_RS25120	90009..90266	-	258	WP_000084404.1	replication regulatory protein RepA	-
I6J89_RS25125	90551..90700	-	150	WP_001336447.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	90748..90804	+	57	NuclAT_1	-	Antitoxin
-	90748..90804	+	57	NuclAT_1	-	Antitoxin
-	90748..90804	+	57	NuclAT_1	-	Antitoxin
-	90748..90804	+	57	NuclAT_1	-	Antitoxin
I6J89_RS25130	90899..91336	-	438	WP_000872609.1	hypothetical protein	-
I6J89_RS25135	91489..91872	-	384	WP_001109264.1	hypothetical protein	-
I6J89_RS25140	91975..92436	-	462	WP_000760080.1	thermonuclease family protein	-
I6J89_RS25145	93189..93392	-	204	WP_001336517.1	hypothetical protein	-
I6J89_RS25150	93544..94101	-	558	WP_000139329.1	fertility inhibition protein FinO	-
I6J89_RS25155	94156..94902	-	747	WP_000205709.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A)	senB	1..144098	144098
	-	flank	IS/Tn	-	-	85629..86006	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T190931 WP_001336447.1 NZ_CP069561:c90700-90551 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T190931 NZ_CP091578:c231043-230771 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT190931 NZ_CP069561:90748-90804 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin