Detailed information of TA system TA190926

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 40764..41006
• Replicon: plasmid unnamed1
• Accession: NZ_CP069560
• Organism: Escherichia coli strain FDAARGOS_1259

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: I6J89_RS24435
• Protein ID: WP_001312861.1
• Coordinates: 40764..40922 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	40966..41006 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J89_RS24405	36185..37045	+	861	WP_000027057.1	class A broad-spectrum beta-lactamase TEM-1	-
I6J89_RS24410	37195..37566	-	372	Protein_40	IS6-like element IS26 family transposase	-
I6J89_RS24415	37716..38318	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
I6J89_RS24420	38615..39436	-	822	WP_001234469.1	DUF945 domain-containing protein	-
I6J89_RS24425	39555..39842	-	288	WP_000107535.1	hypothetical protein	-
I6J89_RS24430	39867..40073	-	207	WP_000275859.1	hypothetical protein	-
I6J89_RS24435	40764..40922	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	40966..41006	-	41	NuclAT_1	-	Antitoxin
-	40966..41006	-	41	NuclAT_1	-	Antitoxin
-	40966..41006	-	41	NuclAT_1	-	Antitoxin
-	40966..41006	-	41	NuclAT_1	-	Antitoxin
-	42450..42636	-	187	NuclAT_0	-	-
-	42450..42636	-	187	NuclAT_0	-	-
-	42450..42636	-	187	NuclAT_0	-	-
-	42450..42636	-	187	NuclAT_0	-	-
I6J89_RS24445	42648..43367	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
I6J89_RS24450	43364..43798	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
I6J89_RS24455	43853..45811	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2 / blaOXA-9 / blaTEM-1A	-	1..67959	67959
	-	inside	IScluster/Tn	blaKPC-2 / blaOXA-9 / blaTEM-1A	-	22682..42402	19720

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T190926 WP_001312861.1 NZ_CP069560:c40922-40764 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T190926 NZ_CP091575:c56447-56166 [Salmonella enterica]
ATGATTTATGAACTGGCATTTGACCACAGAGCACTGAAGGAATGGCAAAAACTCGGTCACACTGTTCGTGAGCAATTCAA
AAAGAAACTGTCAGAACGCCTGATAAATCCACGCGTCCCCGCAGCCAAGCTACATGGTCATACTGACCGTTACAAAATTA
AGCTTCGCGCATCCGGTTACAGGCTCGTTTATCAGGTAATTGATGAGAAGGTCGTTTTGCTTGTGATTTCTGTGGGAAAA
AGAGAAGGCAGCGACGTCTATCACATGGCAGATACCCGCTGA

Antitoxin

Nucleotide

• Length: 41 bp

>AT190926 NZ_CP069560:c41006-40966 [Escherichia coli]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin