Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2868083..2868645 Replicon chromosome
Accession NZ_CP069559
Organism Escherichia coli strain FDAARGOS_1259

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag I6J89_RS13730 Protein ID WP_000605675.1
Coordinates 2868083..2868361 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag I6J89_RS13735 Protein ID WP_000781370.1
Coordinates 2868361..2868645 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J89_RS13705 2863670..2864101 - 432 WP_000152305.1 peroxiredoxin OsmC -
I6J89_RS13710 2864446..2864661 + 216 WP_000495766.1 biofilm-dependent modulation protein -
I6J89_RS13715 2864763..2864900 + 138 WP_099989695.1 stationary-phase-induced ribosome-associated protein -
I6J89_RS13720 2865056..2866753 + 1698 WP_000433462.1 malate dehydrogenase -
I6J89_RS13725 2866887..2867897 + 1011 WP_045232988.1 alcohol dehydrogenase AdhP -
I6J89_RS13730 2868083..2868361 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6J89_RS13735 2868361..2868645 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
I6J89_RS13740 2868679..2870025 - 1347 WP_000483766.1 IS4-like element IS4 family transposase -
I6J89_RS13745 2870312..2870965 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
I6J89_RS13750 2870958..2871842 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2868679..2870007 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T190913 WP_000605675.1 NZ_CP069559:2868083-2868361 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T190913 NZ_CP091571:2136018-2136121 [Salmonella enterica]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT190913 WP_000781370.1 NZ_CP069559:2868361-2868645 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT190913 NZ_CP091571:c2136123-2135978 [Salmonella enterica]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References