Detailed information of TA system TA190902

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1600178..1600905
• Replicon: chromosome
• Accession: NZ_CP069559
• Organism: Escherichia coli strain FDAARGOS_1259

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: I6J89_RS07615
• Protein ID: WP_000547563.1
• Coordinates: 1600178..1600489 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6J89_RS07620
• Protein ID: WP_000126304.1
• Coordinates: 1600486..1600905 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J89_RS07585	1595322..1597031	+	1710	WP_001288123.1	formate hydrogenlyase subunit HycE	-
I6J89_RS07590	1597041..1597583	+	543	WP_000493800.1	formate hydrogenlyase subunit HycF	-
I6J89_RS07595	1597583..1598350	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
I6J89_RS07600	1598347..1598757	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
I6J89_RS07605	1598750..1599217	+	468	WP_000132955.1	hydrogenase maturation peptidase HycI	-
I6J89_RS07610	1599260..1600015	+	756	WP_001314074.1	hypothetical protein	-
I6J89_RS07615	1600178..1600489	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
I6J89_RS07620	1600486..1600905	+	420	WP_000126304.1	helix-turn-helix domain-containing protein	Antitoxin
I6J89_RS07625	1600997..1601425	-	429	WP_000536066.1	DUF4259 domain-containing protein	-
I6J89_RS07630	1601810..1602337	+	528	WP_001078777.1	electron transport protein HydN	-
I6J89_RS07635	1602490..1604742	+	2253	WP_001535518.1	carbamoyltransferase HypF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T190902 WP_000547563.1 NZ_CP069559:1600178-1600489 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T190902 NZ_CP091569:c4211477-4211193 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15474.50 Da
• Isoelectric Point: 4.4596

>AT190902 WP_000126304.1 NZ_CP069559:1600486-1600905 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT190902 NZ_CP091569:c4211709-4211467 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22