Detailed information of TA system TA190880

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2325956..2326650
• Replicon: chromosome
• Accession: NZ_CP069549
• Organism: Escherichia coli strain FDAARGOS_1253

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: I6J83_RS11255
• Protein ID: WP_001263500.1
• Coordinates: 2325956..2326354 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: I6J83_RS11260
• Protein ID: WP_000554758.1
• Coordinates: 2326357..2326650 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J83_RS11230	2321321..2321779	-	459	WP_001291991.1	xanthine phosphoribosyltransferase	-
I6J83_RS11235	2322040..2323497	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
I6J83_RS11240	2323554..2324168	-	615	WP_000602123.1	peptide chain release factor H	-
I6J83_RS11245	2324165..2325304	-	1140	WP_000521564.1	RNA ligase RtcB family protein	-
I6J83_RS11250	2325494..2325946	-	453	WP_001059897.1	GNAT family N-acetyltransferase	-
I6J83_RS11255	2325956..2326354	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
I6J83_RS11260	2326357..2326650	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
I6J83_RS11265	2326702..2327757	-	1056	WP_001226162.1	DNA polymerase IV	-
I6J83_RS11270	2327828..2328613	-	786	WP_000207543.1	putative lateral flagellar export/assembly protein LafU	-
I6J83_RS11275	2328585..2330297	+	1713	Protein_2212	flagellar biosynthesis protein FlhA	-
I6J83_RS11280	2330417..2330914	-	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	2325956..2346110	20154

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T190880 WP_001263500.1 NZ_CP069549:c2326354-2325956 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T190880 NZ_CP069549:c2326354-2325956 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT190880 WP_000554758.1 NZ_CP069549:c2326650-2326357 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT190880 NZ_CP069549:c2326650-2326357 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3